低剂量辐照对人外周血淋巴细胞的影响  

Effects of Low dose Irradiation on Human Peripheral Blood Lymphocytes

作　　者：梁笑星 杨璐 马铭梓 陈兴慧 孙莉萍 郝艳梅 汪德清 LIANG Xiao-xing;YANG Lu;MA Ming-zi(School of Laboratory Medicine,Bengbu Medical College,Bengbu,Anhui 233030)

机构地区：[1]蚌埠医学院检验医学院,233030 [2]中国人民解放军总医院第一医学中心输血医学科 [3]沈阳市妇婴医院输血科

出　　处：《临床输血与检验》2023年第1期75-81,共7页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：联保部队重点学科专业建设(No.A350109)资助。

摘　　要：目的研究人外周血中淋巴细胞亚群对相同辐照剂量下不同辐照次数处理模式时的辐照敏感性差异,为免疫性疾病治疗提供一种新思路。方法采集志愿者外周血,分离提取外周血淋巴细胞,用总剂量为300 mGy分别辐照6次(50 mGy/次)、3次(100 mGy/次)、2次(150 mGy/次),每次照射间隔24 h,每个剂量最后一次照射后24 h检测凋亡、增殖、周期、细胞因子。结果与对照比较,经照射处理后的淋巴细胞凋亡率明显增加;在50 mGy/次照射剂量组中CD3^(-)CD56^(+)NK>CD3^(+)T>CD3^(-)CD19^(+)B细胞,细胞因子分泌量呈现上升趋势;100 mGy、150 mGy/次照射剂量组中CD3^(-)CD56^(+)NK>CD3^(-)CD19^(+)B>CD3^(+)T细胞,细胞因子分泌呈现下降趋势。50 mGy、100 mGy和150 mGy/次的X射线照射处理后,淋巴细胞增殖活性受到抑制,阻滞在G0/G1期。结论相同剂量不同辐照次数的低剂量辐照诱导凋亡处理模式对人外周血淋巴细胞亚群呈现不同的辐照敏感性,可抑制部分淋巴细胞的增殖活性和细胞因子分泌功能,阻滞淋巴细胞在G0/G1期。Objective To study the difference of radiosensitivity of lymphocyte subsets in human peripheral venous blood to different irradiation times under the same irradiation dose,and provide a new idea for the treatment of immune diseases.Methods The peripheral blood of volunteers was collected,the peripheral blood lymphocytes were isolated and extracted,and irradiated 6 times(50 mGy/time),3 times(100 mGy/time),and 2 times(150 mGy/time)with a total dose of 300 mGy.The intervals between each irradiation were 24 h,and apoptosis,proliferation,cycle,and cytokines were detected 24 h after the last irradiation of each dose.Results Compared with the control,the apoptosis rate of lymphocytes after irradiation was significantly increased.CD3^(-)CD56^(+)NK>CD3^(+)T>CD3^(-)CD19^(+)B cells in the 50 mGy/time group;CD3^(-)CD56^(+)NK>CD3^(-)CD19^(+)B>CD3^(+)T cells in 100 mGy and 150 mGy/times groups.After 50 mGy,100 mGy and 150 mGy/time X-ray irradiation,the proliferation activity of lymphocytes was inhibited;Lymphocytes were blocked in G0/G1 phase.Conclusion The same dose and different low-dose irradiation induced apoptosis treatment mode showed different radiosensitivity to human peripheral blood lymphocyte subsets,which could inhibit the proliferation activity and cytokine secretion function of some lymphocytes,and block lymphocytes in G0/G1 phase.

关 键 词：低剂量辐照 人外周血淋巴细胞 辐照敏感性 

分 类 号：R144[医药卫生—公共卫生与预防医学]