雪茄烟中霉菌计数方法的适用性评价  

Evaluating the applicability of mould counting methods for cigar

作　　者：黄阔 叶长文[1] 李东亮 李青常[1] 朱贝贝 刘萌萌 范黎[1] 史占东 薛芳 李栋[1] HUANG Kuo;YE Changwen;LI Dongliang;LI Qingchang;ZHU Beibei;LIU Mengmeng;FAN Li;SHI Zhandong;XUE Fang;LI Dong(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;China Tobacco Sichuan Industrial Co.,Ltd.,Chengdu 610066,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]四川中烟工业有限责任公司,成都市610066

出　　处：《烟草科技》2023年第1期39-46,共8页Tobacco Science & Technology

基　　金：中国烟草总公司郑州烟草研究院青年人才托举工程计划项目“手工雪茄烟霉变拮抗微生物的筛选和应用研究”(702021CR0090);中国烟草总公司重大科技项目“国产手工雪茄养护醇化关键技术创新与应用研究”[110202101066(XJ-15)];四川中烟工业有限责任公司科技项目“手工雪茄烟加工过程霉变微生物分析及监控研究”(XJ202109)。

摘　　要：为明确霉菌计数方法对雪茄烟的适用性,采用孟加拉红琼脂培养基、改良马铃薯葡萄糖琼脂培养基和SYMPHONY Agar快速显色培养基对13种雪茄烟进行霉菌计数,评价3种培养基在不同接种方式条件下雪茄烟中霉菌计数结果的一致性,并对各霉菌计数方法进行比较。结果表明:(1)同种接种方式条件下,3种培养基的霉菌计数结果的一致性较好;(2)相同培养基条件下,平板涂布法操作更简便,菌落分布均匀,典型菌落更易判读;(3)3种霉菌计数培养基相比,SYMPHONY Agar快速显色培养基培养周期短,霉菌典型菌落大小均匀,更容易计数。因此,SYMPHONY Agar快速显色培养基和平板涂布法接种方式更适合于雪茄烟中的霉菌计数。To investigate the applicability of mould counting methods for cigar,the degrees of mould in13 cigar samples were counted by using rose bengal agar medium,modified potato dextrose agar medium,and SYMPHONY Agar rapid chromogenic medium.The consistency of the mould counting results on the three media with different inoculation methods was evaluated,and the mould counting methods were compared.The results showed that:1)The consistency of the mould counting results on the three media was better when adopting the same inoculation method.2)On the same medium,spread plate method was more convenient in operation,the colonies distributed evenly,and the typical colonies were easier to be interpreted.3)Comparing with the other two media,SYMPHONY Agar rapid chromogenic medium required a shorter culture period,the sizes of typical mould colonies were uniform,and the mould counting was easier.Therefore,SYMPHONY Agar rapid chromogenic medium combined with the spread plate method is more suitable to the mould counting of cigar.

关 键 词：雪茄烟 霉菌计数方法 培养基 平板涂布法 平板倾注法 

分 类 号：TS414[农业科学—烟草工业]