β-氨基丁酸对烟草黑胫病的抗性诱导  

Resistance induction byβ-aminobutyric acid against tobacco black shank disease

作　　者：任锡跃 刘涛[1,2,3] 朱发亮 王志江 叶贤文[4] 梅坚[4] 王建军 REN Xiyue;LIU Tao;ZHU Faliang;WANG Zhijiang;YE Xianwen;MEI Jian;WANG Jianjun(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;National-Local Joint Engineering Research Center on Germplasm Innovation&Utilization of Chinese Medicinal Materials in Southwest China,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Medicinal Plant Biology of Yunnan Province,Yunnan Agricultural University,Kunming 650201,China;Kunming Branch of Yunnan Provincial Tobacco Company,Kunming 650051,China)

机构地区：[1]云南农业大学农学与生物技术学院,昆明市650201 [2]云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,昆明市650201 [3]云南农业大学云南省药用植物生物学重点实验室,昆明市650201 [4]云南省烟草公司昆明市公司,昆明市650051

出　　处：《烟草科技》2023年第1期47-51,65,共6页Tobacco Science & Technology

基　　金：云南中烟工业有限责任公司项目“云南中烟重点品牌主导规格产品提质维护技术体系构建”(KX141498000)。

摘　　要：为明确β-氨基丁酸(β-aminobutyric acid,BABA)对烟草由寄生疫霉(Phytophthora parasitica)引起的黑胫病抗性的诱导效果,对BABA处理后的烟草进行了病害调查,并检测了生理生化指标和抗性相关基因表达量。结果表明:(1)BABA诱导烟草产生超敏反应(Hypersensitive response,HR),且诱导与产生超敏反应相关的HIN1基因表达量增加。(2)BABA可诱导过氧化物酶(Peroxidase,POD)、多酚氧化酶(Polyphenol oxidase,PPO)和过氧化氢酶(Catalase,CAT)活性提高;在烟草未接种P.parasitica时,BABA不仅诱导O_(2)^(-)含量增加了142.2%,还导致丙二醛(Malondialdehyde,MDA)的积累;烟草接种P.parasitica后,BABA使与活性氧产生相关的NtrbohD基因表达量降低56.9%。(3)5 mmol/L BABA处理对烟草的黑胫病抗性具有诱导作用,处理后烟草黑胫病的防治效果达56.3%。To clarify the resistance induction effect of β-aminobutyric acid(BABA)against tobacco black shank disease caused by Phytophthora parasitica,the disease investigation was carried out for tobacco treated by BABA,the physiological and biochemical indexes and expression levels of resistance related genes were detected.The results showed that:1)BABA induced hypersensitive response(HR)and increased the expression of HIN1 gene which was related to HR in tobacco.2)BABA promoted the activities of peroxidase(POD),polyphenol oxidase(PPO)and catalase(CAT).When tobacco was not inoculated with P.parasitica,BABA not only induced a significant increase of O_(2)^(-)content by 142.2%,but also caused the accumulation of malondialdehyde(MDA).After tobacco was inoculated with P.parasitica,BABA reduced the expression level of NtrbohD which was involved in the production of reactive oxygen by 56.9%.3)The treatment of 5 mmol/L BABA had a resistance induction effect for tobacco against black shank disease,and the control effect was 56.3%after the treatment.

关 键 词：烟草黑胫病 Β-氨基丁酸 抗性诱导 HIN1基因 

分 类 号：S47[农业科学—农业昆虫与害虫防治]