环境浓度的丁草胺暴露对雌性褐菖鲉卵巢发育的干扰  

Interference of butachlor exposure at environmental concentration on ovarian development of female Sebastiscus marmoratus

作　　者：郭团玉 李进寿 何亮银 罗芬 阮峻峰 周逢芳 GUO Tuanyu;LI Jinshou;HE Liangyin;LUO Fen;RUAN Junfeng;ZHOU Fengfang(Ningde Normal University,Ningde 352100,China;Xiamen Marine Vocational and Technical College,Xiamen,361100,China)

机构地区：[1]宁德师范学院生命科学学院,福建宁德352100 [2]厦门海洋职业技术学院海洋生物学院,福建厦门361100

出　　处：《应用海洋学学报》2023年第1期49-55,共7页Journal of Applied Oceanography

基　　金：福建省自然科学基金资助项目(2020J01426);宁德师范学院科技特派员科研专项资助项目(2022ZQ401);宁德师范学院中青年科研资助项目(2022ZQ101)。

摘　　要：丁草胺是使用最广泛的酰胺类除草剂之一,目前丁草胺对鱼类的生殖毒性研究集中于淡水种类,近海鱼类鲜有报道。本研究以分布于我国东南海域的近海经济鱼类褐菖鲉(Sebastiscus marmoratus)为受试对象,探讨丁草胺对海洋鱼类卵巢发育的影响及机制。褐菖鲉在以环境相关浓度(2、20、200 ng/L)的丁草胺暴露50 d后,褐菖鮋卵巢生殖细胞发育受到不同程度抑制,卵巢17β-雌二醇(E2)水平下降,Caspase-3活性上升。相对定量PCR分析结果显示,卵巢芳香化酶基因(CYP 19 s)及雌激素受体基因(ERβ)表达量下降。结果表明:丁草胺对褐菖鮋具有明显的生殖毒性,卵巢CYP 19 s基因与ERβ基因表达被抑制而导致卵巢雌激素水平的下降引起卵细胞在发育早期的细胞凋亡是主要原因。Butachlor is one of the most widely used amide herbicides.Although the researches on the reproductive toxicity of butachlor to freshwaterfish have been reported extensively,few studies addressing the reproductive toxicity of butachlor on the offshore fishes are available.The present study was conducted to explore the effects of butachlor on ovarian development in Sebastiscus marmoratus,a common offshore fish distributes in the southeast coast of China,and to gain insight into its mechanism.After exposing to butachlor at environmental concentrations(2,20 and 200 ng/L)for 50 d,the development of ovarian germ cell was depressed to different extents.While 17βestradiol(E2)was decreased in the ovaries,the activity of Caspase-3 increased.Real-time PCR showed that the expressions of CYP 19 s and ERβwere down-regulatedin the ovaries.These results indicated that butachlor can cause significant reproductive toxicity to S ebastiscus marmoratus,and the inhibited expression of CYP 19 s gene and ERβin the ovary leads to the decrease of estrogen level and apoptosis of egg cells in early development.

关 键 词：海洋生物学 丁草胺 生殖毒性 褐菖鲉 细胞凋亡 卵巢 

分 类 号：P735[天文地球—海洋生物学]