SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana  

作　　者：Xilong Li Zongyun Yan Meiling Zhang Jiayin Wang Peiyong Xin Shujing Cheng Liquan Kou Xiaoting Zhang Songlin Wu Jinfang Chu Chengqi Yi Keqiong Ye Bing Wang Jiayang Li 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,China [3]Key Laboratory of RNA Biology,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [4]University of Chinese Academy of Sciences,Beijing 100039,China [5]Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China

出　　处：《Science China(Life Sciences)》2023年第1期2-11,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(31788103,32122012,32100221,91940302,31430024);the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24040202);the Chinese Academy of Sciences Youth Innovation Promotion Association(2019099);the CAS Key Technology Talent Program(2017)。

摘　　要：Polyamines have been discovered for hundreds of years and once considered as a class of phytohormones.Polyamines play critical roles in a range of developmental processes.However,the molecular mechanisms of polyamine signaling pathways remain poorly understood.Here,we measured the contents of main types of polyamines,and found that endogenous level of thermospermine(T-Spm)in Arabidopsis thaliana is comparable to those of classic phytohormones and is significantly lower than those of putrescine(Put),spermidine(Spd),and spermine(Spm).We further found a nodule-like structure around the junction area connecting the shoot and root of the T-Spm biosynthetic mutant acl5 and obtained more than 50 suppressors of acl5 nodule structure(san)through suppressor screening.An in-depth study of two san suppressors revealed that NAP57 and NOP56,core components of box H/ACA and C/D snoRNPs,were essential for T-Spm-mediated nodule-like structure formation and plant height.Furthermore,analyses of rRNA modifications showed that the overall levels of pseudouridylation and 2′-O-methylation were compromised in san1 and san2 respectively.Taken together,these results establish a strong genetic relationship between rRNA modification and T-Spm-mediated growth and development,which was previously undiscovered in all organisms.

关 键 词：polyamines thermospermine SNORNP pseudouridylation 2’-O-methylation Arabidopsis thaliana 

分 类 号：Q941[生物学—植物学]