水稻细菌性谷枯病菌和水稻白叶枯病菌荧光RPA快速检测方法的建立  

Establishment of fluorescence RPA detection methods for Burkholderia glumae and Xanthomonas oryzae pv. oryzae

作　　者：方雪阳 田茜[2] 孙羽佳 罗来鑫[1] 赵文军[2] 王辉 Fang Xueyang;Tian Qian;Sun Yujia;Luo Laixin;Zhao Wenjun;Wang Hui(China Agricultural University,Beijing100193,China;Chinese Academy of Inspectionand Quarantine;Hainan Provincial Administration of Southern Propagation)

机构地区：[1]中国农业大学,北京100193 [2]中国检验检疫科学研究院 [3]海南省南繁管理局

出　　处：《植物检疫》2023年第1期49-55,共7页Plant Quarantine

基　　金：海南省重点研发计划(ZDYF2020088);中国检验检疫科学研究院基本科研业务费项目(2022JK10)。

摘　　要：本研究分别针对我国进境植物检疫性有害生物水稻细菌性谷枯病菌及水稻白叶枯病菌建立了光RPA检测方法,并对其灵敏度、特异性以及对实际种子样本的检测能力进行了测试评价。结果表明,本研究建立的两种方法均能够在20 min内特异性检测到目标菌株,对水稻细菌性谷枯病菌(Burkholderia glumae)菌液的检测下限达到了11.4 CFU/反应,DNA检测下限达到了4.83×10^(-5)ng/反应;对水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae)菌液的检测下限达到了4.42 CFU/反应,DNA检测下限达到了3.83×10^(-4)ng/反应。两种方法均能够成功应用于带菌种子样品的快速检测。Two fluorescence RPA methods for detection of quarantine bacterium Burkholderia glumae and Xanthomonas oryzae pv.oryzae were developed respectively.The sensitivity,specificity and the ability to detect actual seed samples of these two methods were evaluated.The detection results suggested that the target strains could all be detected within 20 min using the RPA methods established in this study.The detection threshold of Burkholderia glumae is 11.4 CFU/reaction for bacteria culture and 4.83×10^(-5)ng/reaction for DNA.While the detection threshold of Xanthomonas oryzae pv.oryzae is 4.42 CFU/reaction for bacteria culture and 3.83×10^(-4)ng/reaction for DNA.Both methods can be successfully applied to the rapid detection of infected seed samples.

关 键 词：水稻白叶枯病菌 水稻细菌性谷枯病菌 荧光RPA 快速检测 

分 类 号：S41[农业科学—植物保护]