电针对多囊卵巢综合征小鼠机械痛阈及TRPV1和CGRP表达的影响  

Effects of electroacupuncture on mechanical pain threshold and expression of TRPV1 and CGRP in mice with polycystic ovary syndrome

作　　者：徐晨曦 胡瑞斌 高昕妍 乔海法[1,2,3] 袁伟 XU Chenxi;HU Ruibin;GAO Xinyan;QIAO Haifa;YUAN Wei(College of Acupuncture&Tuina,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Shaanxi Key Laboratory of Acupuncture&Medicine,Xianyang 712046,China;Xianyang Key Laboratory of Neurobiology(Acupuncture),Xianyang 712046,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]陕西中医药大学针灸推拿学院,咸阳712046 [2]陕西省针药结合重点实验室,咸阳712046 [3]咸阳市神经生物学(针灸)重点实验室,咸阳712046 [4]中国中医科学院针灸研究所,北京100700

出　　处：《中国疼痛医学杂志》2023年第1期12-19,共8页Chinese Journal of Pain Medicine

基　　金：陕西中医药大学经脉-脏腑相关研究创新团队(No.YL-09);陕西中医药大学博士科研启动金项目(No.303-17102031914);陕西中医药大学校级科研课题(No.2021GP35)。

摘　　要：目的:观察电针关元及敏化点对多囊卵巢综合征(polycystic ovary syndrome,PCOS)小鼠关元穴、敏化点机械痛阈值及T13-L2节段脊髓、背根神经节(dorsal root ganglion,DRG)中瞬时受体电位香草酸亚型1(transient receptor potential vanilloid 1,TRPV1)、降钙素基因相关肽(calcitonin gene related peptide,CGRP)表达的影响,探讨PCOS小鼠模型体表敏化的部分机制。方法:32只雌性ICR小鼠,随机分为对照组、模型组、电针关元组、电针敏化点组,每组8只。采用双酚A灌胃的方式制备PCOS模型,对照组用玉米油灌胃;电针关元及敏化点,每日20 min,连续5天,共4周。电针结束后第3天,用von Frey测量小鼠体表敏化点及关元穴机械痛阈值;HE染色法观察卵巢组织病理形态;蛋白印迹法、免疫荧光法检测脊髓、DRG中TRPV1及CGRP表达水平。结果:与对照组比较,模型组卵巢组织囊性扩张、闭锁卵泡增加,关元穴、敏化点痛阈值下降,脊髓及DRG中TRPV1、CGRP蛋白表达上升,脊髓中TRPV1/CGRP共表达光密度升高、DRG中TRPV1/CGRP共表达细胞增加;与模型组比较,电针关元组及电针敏化点组卵泡及黄体发育良好,关元穴及敏化点痛阈值升高,脊髓、DRG中TRPV1及CGRP蛋白表达降低、脊髓中TRPV1/CGRP共表达光密度降低、DRG中TRPV1/CGRP共表达细胞减少。结论:电针敏化点与关元可提高PCOS小鼠关元穴及敏化点机械痛阈值,调节PCOS小鼠体表敏化现象,其作用机制可能与下调TRPV1、CGRP的异常高表达有关。Objective:To observe the effects of electroacupuncture(EA)at Guan Yuan(CV4)and sensitization point on the mechanical pain threshold of CV4 acupoint and sensitization point and the expression of transient receptor potential vanilloid 1(TRPV1)and calcitonin gene related peptide(CGRP)in T13-L2segment spinal cord and dorsal root ganglion(DRG)in mice with polycystic ovary syndrome(PCOS),and to explore partial mechanisms of PCOS mice model body surface sensitization.Methods:Thirty-two female ICR mice were randomly divided into control group,model group,electroacupuncture(EA)Guan yuan(CV4)group and EA-sensitization group,with 8 mice in each group.PCOS mice models were intragastrically administered with bisphenol A,and the control group was intragastrically administered with corn oil.EA-CV4 and EA-sensitization point,20 min a day,for 5 consecutive days,a total of 4 weeks.On the third day after the EA,the mechanical pain threshold of the sensitized point on the body surface and CV4 were measured by von Frey.HE staining was used to observe the pathological morphology of mice ovarian tissue.The expression levels of TRPV1 and CGRP in spinal cord and DRG tissues were detected by Western blot and Immunofluorescence.Results:Compared with the control group,the cystic dilatation and atresia of ovarian tissue were increased in the model group,and the mechanical pain threshold of CV4 acupoint and sensitized point were decreased,the protein expressions of TRPV1 and CGRP in the spinal cord and DRG tissue were increased,the optical density of TRPV1/CGRP co-expression in spinal cord was increased,and the number of TRPV1/CGRP co-expressing cells in the DRG was increased.Compared with model group,the follicles and luteum of the EA-CV4 group and the EA-sensitization group were well developed,the mechanical pain threshold of CV4 acupoint and sensitized point increased,the protein expression of TRPV1 and CGRP in spinal cord and DRG tissues were decreased,the optical density of TRPV1/CGRP co-expression in the spinal cord were decreased,the T

关 键 词：多囊卵巢综合征 穴位敏化 机械痛阈 瞬时受体电位香草酸亚型1 降钙素基因相关肽 

分 类 号：R245[医药卫生—针灸推拿学]