应用磁共振波谱研究针刺对健康人前额叶神经递质影响的性别差异  

Magnetic resonance spectroscopy study on gender differences in the effects of acupuncture on prefrontal neurotransmitters in healthy volunteers

作　　者：赵彦萍[1] 方继良[1] 陈媛媛[1] 王寅[1] 李小娇[1] 孙黎[1] 史珊[1] 孙继飞 马跃 王智 郭春蕾 罗屹 张国雷 洪洋[1] 罗萍[1] ZHAO Yanping;FANG Jiliang;CHEN Yuanyuan;Wang Yin;LI Xiaojiao;SUN Li;SHI Shan;SUN Jifei;MA Yue;WANG Zhi;GUO Chunlei;LUO Yi;ZHANG Guolei;Hong Yang;LUO Ping(Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《上海针灸杂志》2023年第2期211-217,共7页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目(81273674,82174282);科技部重点研发计划(2018YFC1705802);中国中医科学院广安门医院所级科研基金(2016s355);中国中医科学院科技创新工程(461092)。

摘　　要：目的采用磁共振波谱MEGA-PRESS序列,观察针刺合谷穴对不同性别健康人前额叶内神经递质谷氨酸(glutamate,Glu)、谷氨酰胺(glutamine,Glx)及r-氨基丁酸(γ-aminobutyric acid,GABA)浓度变化的影响。方法分别采集76例健康受试者刺激前及刺激(手针和纤毛针)合谷穴时磁共振波谱(MRS)数据,采用t检验及Pearson统计,分析刺激前后Glu^(+)、Glx^(+)和GABA^(+)浓度变化的性别差异及相关性。结果手针刺激前男性Glu^(+)浓度明显高于女性(P<0.05),手针刺激时男性Glu^(+)和Glx^(+)浓度均明显高于女性(P<0.05);纤毛针刺激前男性Glx^(+)浓度明显高于女性(P<0.05),纤毛针刺激时男性Glu^(+)和Glx^(+)浓度均明显高于女性(P<0.05);但手针和纤毛针刺激前和刺激时男性GABA^(+)浓度与女性比较,差异无统计学意义(P>0.05)。手针刺激前和刺激时,性别与Glu^(+)及Glx^(+)浓度均呈正相关(P<0.05);纤毛针刺激前和刺激时,男性与Glu^(+)及Glx^(+)浓度呈正相关(P<0.05);手针和纤毛针刺激前和刺激时,性别与GABA^(+)浓度均无明显相关性(P>0.05)。结论大脑前额叶内兴奋性神经递质(Glu和Glx)浓度可能存在性别差异,针刺合谷穴对大脑兴奋性神经递质浓度的影响可能存在性别差异。Objective To study the gender differences in the impact of acupuncture at Hegu(LI4)on the concentrations of neurotransmitters glutamate(Glu),glutamine(Glx),andγ-aminobutyric acid(GABA)in the prefrontal cortex using magnetic resonance spectroscopy(MRS)MEGA-PRESS sequence.Method MRS data were collected from 76 healthy volunteers before and during stimulating Hegu point(using manual acupuncture or von Frey filament).Gender differences in the concentration changes of Glu^(+),Glx^(+),and GABA^(+)and their interactions were analyzed by t-test and Pearson test.Result The concentration of Glu^(+)was significantly higher in males than in females before manual acupuncture stimulation(P<0.05),and the concentrations of both Glu^(+)and Glx^(+)were significantly higher in males than in females during manual acupuncture stimulation(P<0.05).The concentration of Glx^(+)was notably higher in males than in females before von Frey filament stimulation(P<0.05),and the concentrations of both Glu^(+)and Glx^(+)were significantly higher in males than in females during von Frey filament stimulation(P<0.05).There was no significant difference in the concentration of GABA^(+)between the two genders before or during either stimulation(P>0.05).Before and during manual acupuncture stimulation,there was a positive correlation between gender and the concentrations of Glu^(+)and Glx^(+)(P<0.05).Before and during von Frey filament stimulation,there was a positive correlation between the male gender and the concentrations of Glu^(+)and Glx^(+)(P<0.05).There was no significant correlation between gender and the concentration of GABA^(+)before or during either stimulation(P>0.05).Conclusion There are potential gender differences in the concentrations of excitatory neurotransmitters(Glu and Glx)in the prefrontal cortex,and the impact of acupuncture at Hegu point on the concentrations of brain excitatory neurotransmitters may also vary between genders.

关 键 词：针刺疗法 穴 合谷 磁共振波谱 谷氨酸 谷氨酰胺 Γ-氨基丁酸 性别 

分 类 号：R245[医药卫生—针灸推拿学]