梨胶锈菌侵染西府海棠和山荆子转录组比较  

Comparative Transcriptome of Malus micromalus and Malus baccata Infected by Gymmosporangium haraeanum

作　　者：王学府[1] 狄红梅[1] 曹秋芬 Wang Xuefu;Di Hongmei;Cao Qiufen(Jinzhong Vocational&Technical College,Department of Biotechnology Jinzhong,030602;College of Life Science,Shanxi Agricultural University,Taiyuan,030002)

机构地区：[1]晋中职业技术学院生物工程系,晋中030602 [2]山西农业大学生命科学学院,太原030002

出　　处：《分子植物育种》2023年第3期754-763,共10页Molecular Plant Breeding

基　　金：山西省重点研发计划项目(201803D221003-8)资助。

摘　　要：研究苹果属西府海棠和山荆子在梨胶锈菌侵染下的生理和分子响应机制,发掘抗病相关的功能基因,对研究苹果属植物的抗病分子机制有重要意义。对西府海棠及山荆子分别接种梨胶锈菌,进而对接种锈菌前后的西府海棠和山荆子进行高通量转录组测序,并利用生物信息学方法对基因表达和功能进行分析。离体叶片抗病性鉴定表明,山荆子对梨胶锈菌的抗性最强,西府海棠抗性最弱;高通量测序表明,有74.73%~80.61%的clean数据比对到唯一的基因组位点,说明测序质量好,可信度高;采用RPKM(Reads Per Kilobase of exon model per Million mapped reads,每百万读段中来自于某个基因每千碱基上映射的平均读段数)法计算基因表达量,共筛选出山荆子和西府海棠接种梨胶锈菌后的1185个差异表达基因。其中687个差异基因在基因本体联合会数据库(gene ontology,GO)中富集,主要功能有酶活性、代谢过程和防御反应等。有320个差异基因能归入京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路,包括植物病原体相互作用、植物激素信号转导、泛素化信号通路等过程。其中,在13个富集在植物病原体互作通路的差异基因中,有5个表现为钙调素类蛋白功能,说明在钙信号通路的活跃是山荆子相较西府海棠对锈病有较强抗性的重要原因。山荆子和西府海棠抗锈病是一个高度复杂、多途径协同的过程,由蛋白质代谢、防御反应、激素调节、泛酸化等通路共同调控,其结果可为后期深入研究苹果属抗病分子机制奠定基础,为抗病育种提供理论依据。The objective of this study was to study the physiological and molecular response mechanisms of Malus genus plants of Malus micromalus and Malus baccata infected by Gymmosporangium haraeanum,and to explore the functional genes related to disease resistance,which is of great significance to study the disease resistance mechanisms of Malus genus plants.We inoculated M.micromalus and M.baccata with G.haraeanum,and then carried out high-throughput transcriptome sequencing on M.micromalus and M.baccata before and after inoculation with G.haraeanum,and analyzed the gene expression and function by bioinformatics method.In vitro leaf disease resistance evaluation showed that the resistance of M.baccata was the strongest,and that of M.micromalus was the weakest.High throughput sequencing showed that 74.73%~80.61%of clean data were compared to the unique genome locus,indicating good sequencing quality and high reliability.The gene expression level was calculated by RPKM(Reads Per Kilobase of exon model per Million mapped reads)method,and 1185 differentially expressed genes were screened after inoculation of M.baccata and M.micromalus.Among them,687 differential genes were enriched in GO(Gene Ontology),whose main functions were enzyme activity,metabolic process and defense response.There are 320 DEGs could be included in the KEGG(Kyoto Encyclopedia of Genes and Genomes)pathways,including plant pathogen interaction,plant hormone signal transduction,ubiquitination signal pathway and other processes.Among the 13 differentially enriched genes in plant pathogen interaction pathways,5 showed calmodulin-like protein function,indicating that the activity in calcium signaling pathway is an important reason for the stronger resistance of M.baccata to G.haraeanum compared with M.micromalus.G.haraeanum resistance of M.baccata and M.micromalus is a highly complex and multi-pathway process,which is jointly regulated by protein metabolism,defense response,hormone regulation,panacidification and other pathways.The results can lay a foundat

关 键 词：西府海棠 梨胶锈菌 转录组 差异表达基因 功能 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]