早世代优质小麦的快速筛选鉴定  

Rapid Screening and Identification of Wheat Lines with High Quality in the Earlier Generations

作　　者：王伟[1,2] 闫美[3] 薛立霞 杨在东 程敦公 刘爱峰[1] 宋健民[1] 刘建军[1] 李豪圣[1] 郭军[1] Wang Wei;Yan Mei;Xue Lixia;Yang Zaidong;Cheng Dungong;Liu Aifeng;Song Jianmin;Liu Jianjun;Li Haosheng;Guo Jun(Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley(Ministry of Agriculture),National Engineering Laboratory for Wheat and Maize,Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,250100;Yantai University,Yantai,264005;Shandong Luyan Agricultural Co.,Ltd.,Jinan,250100;Sunwu Street Office and Agro-technical Station,Huimin,251700)

机构地区：[1]山东省农业科学院作物研究所,小麦玉米国家工程实验室,农业部黄淮北部小麦生物学与遗传育种重点实验室,济南250100 [2]烟台大学,烟台264005 [3]山东鲁研农业良种有限公司,济南250100 [4]惠民县孙武街道办事处农技站,惠民251700

出　　处：《分子植物育种》2023年第3期891-895,共5页Molecular Plant Breeding

基　　金：山东省农业良种工程项目(2019LZGC001);山东省农业科学院院创新工程项目(CXGC2018E01);国家重点研发计划项目(2017YFD0100600)共同资助。

摘　　要：为提高育种后代优质小麦新品种的中选概率,本研究以强筋小麦品种‘济麦20’与高产小麦品种‘济麦22’杂交和回交获得的76个BC1F2:4群体为试验材料,通过SDS沉降值测定、面筋指数测定和高分子量谷蛋白亚基电泳分析,筛选到2个优质小麦株系(6225和6294);初步建立了育种后代早世代优质小麦的快速筛选鉴定方法体系,即首先测定株系的面筋指数,再测定谷蛋白亚基组成和SDS沉降值,最后测定稳定时间等品质指标。本研究建立的优质小麦快速筛选方法体系,对于提高优质小麦新品种中选概率具有一定的应用价值。To improve the probability of wheat lines selected with good quality in the breeding programme,76 BC1F2:4linespopulations were obtained by crosscrossing and backcrossing between strong gluten wheat variety’Jimai20’and high yield wheat variety’Jimai22’.Then 2 wheat lines,6225 and 6294 were selected by SDS sedimentation(SDSS)detection,gluten index(GI)detection and high molecular weight glutenin subunits sodium dodecyl sulfate polyacrylamide gel electrophoresis(HMW-GS SDS-PAGE).A rapid screening and identification method system for early generation and high quality wheat was established.Firstly,gluten index was determined,then gluten subunit composition and SDS settlement value were determined.Finally,stability time and other quality indexes were determined.The rapid screening method system of high quality wheat established in this study has certain application value for improving the selection probability of new varieties of high quality wheat.

关 键 词：小麦(Triticum aestivum) 优质 选择方法 

分 类 号：S512.1[农业科学—作物学]