‘宝岛蕉’在盐胁迫下的生理响应与转录组分析  

Physiological Response and Transcriptome Analysis of’Baodao’Banana under Salt Stress

作　　者：魏军亚[1] 刘跃威 魏守兴[1] 程世敏 刘德兵[2] Wei Junya;Liu Yuewei;Wei Shouxing;Cheng Shimin;Liu Debing(Key Tropical Crops Germplasm Utilization,Germplasm Research Institute of Tropical Crops of Chinese Academy of Tropical Agriculture Sciences(Ministry of Agriculture P.R.of China),Danzhou,571737;Applied Science and Technology College,Hainan University,Danzhou,571737)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所国家热带果树品种改良中心,儋州571737 [2]海南大学应用科技学院,儋州571737

出　　处：《分子植物育种》2023年第3期958-965,共8页Molecular Plant Breeding

基　　金：海南省自然科学基金项目(320RC726);海南省科技项目(ZDYF2022XDNY196);海南省高等学校科学研究项目(Hnky2020ZD-3)共同资助。

摘　　要：为揭示盐胁迫下香蕉的生理特性与转录组变化,以‘宝岛蕉’幼苗为材料,设置盐胁迫和正常(对照)处理,分别测定‘宝岛蕉’叶片相对含水量、叶绿素、可溶性糖、可溶性蛋白、根系活力、MDA、SOD和POD活性,脯氨酸和膜透性等与逆境相关的生理指标的变化,并用转录组测序的方法对差异表达基因进行GO、KEGG等分析。结果显示,盐胁迫下‘宝岛蕉’MDA、SOD和POD变化非常明显,与对照相比,植株受到盐胁迫后的MDA活性升高了2.64倍,SOD活性升高了1.75倍,POD活性升高了1.77倍,而可溶性蛋白、膜透性和可溶性糖含量升高较少,相对含水量和叶绿素含量降低也不显著。转录组分析结果表明,与对照相比,盐胁迫处理样品上调基因1113个,下调基因2265个,对差异基因进行GO注释和功能富集性分析,有3378个差异基因注释到数据库中。KEGG分析结果表明,有691个DEGs分布在19个代谢途径上。盐胁迫条件下共注释到48个差异表达转录因子,包括WRKY、MYB、NAC和bHLH等多种类型。研究结果为候选抗性基因的挖掘和筛选奠定了理论基础。In order to reveal the physiological characteristics and transcriptome changes of banana under salt stress,the seedlings of’Baodao Banana’were treated with salt stress and normal(control)treatment.The changes of physiological indexes related to stress,such as relative water content,chlorophyll,soluble sugar,soluble protein,root activity,MDA,SOD and POD activities,proline and membrane permeability,were measured respectively,The differentially expressed genes were analyzed by GO and KEGG by transcriptome sequencing.The results showed that the changes of MDA,SOD and POD in’Baodao Banana’under salt stress were very obvious.Compared with the control,the MDA activity,SOD activity and POD activity increased by 2.64 times,1.75 times and 1.77 times,while the contents of soluble protein,membrane permeability and soluble sugar increased less,and the relative water content and chlorophyll content decreased not significantly.Transcriptome analysis showed that,compared with the control,1113 genes were up-regulated and 2265 genes were down regulated in salt stress treated samples.GO annotation and functional enrichment analysis were carried out for differential genes,and 3378 differential genes were annotated into the database.KEGG analysis showed that 691 DEGs were distributed in 19 metabolic pathways.A total of 48 differentially expressed transcription factors were annotated under salt stress,including WRKY,MYB,NAC and bHLH.The results laid a theoretical foundation for the mining and screening of candidate resistance genes.

关 键 词：宝岛蕉 盐胁迫 生理特性分析 转录组分析 

分 类 号：S668.1[农业科学—果树学]