基于网络药理学方法分析银黄制剂治疗外感性疾病的作用机制  

Analysis of the Mechanism of Yinhuang Preparations in Treatment of Exogenous Disease Based on Network Pharmacology

作　　者：高进 陈承瑜 刘冬丽 陈飞龙 谭涛 龚春晖 曹晖[1] 朱国元 Gao Jin;Chen Chengyu;Liu Dongli;Chen Feilong;Tan Tao;Gong Chunhui;Cao Hui;Zhu Guoyuan(Jinan University College of Pharmacy,Guangzhou 510632;Increasepharm(Hengqin)Institute Co.,Limited,National Engineering Research Center for Modernization of Traditional Chinese Medicine New DDS Branch,Guangdong Province Engineering Research Center for Aerosol Inhalation Preparation,Zhuhai 519000;Macao Institute for Applied Research in Medicine and Health,State Key Laboratory of Quality Research in Chinede Medicines,Macao University of Science and Technology,Macao 999078,China)

机构地区：[1]暨南大学药学院,广东广州510632 [2]盈科瑞(横琴)药物研究院有限公司,国家中药现代化工程技术研究中心新型递药系统分中心,广东省雾化吸入制剂工程技术研究中心,广东珠海519000 [3]澳门科技大学中药质量研究国家重点实验室,中国澳门药物及健康应用研究院,中国澳门999078

出　　处：《广东化工》2023年第2期46-49,39,共5页Guangdong Chemical Industry

基　　金：广东省粤澳科技创新联合资助项目(2020A050515006);澳门科学技术发展基金项目(0075/2019/AGJ)。

摘　　要：目的:基于网络药理学方法,分析银黄制剂治疗肺、呼吸道炎症等外感性疾病的作用机制。方法:从TCMSP数据库获取银黄制剂活性成分、作用靶点;通过Gencards、OMIM、TTD数据库,以咽炎、扁桃体炎、支气管炎、肺炎检索相关疾病靶点,将药物与疾病的交集靶点导入String平台进行蛋白质相互作用分析,构建PPI网络;构建由Cytoscape3.8.2软件完成的“银黄制剂-活性成分-疾病有效靶点”网络图;最后通过Metascape平台进行基因本体(GO)功能和KEGG通路分析,分析其作用机制。结果:发现银黄制剂的222个有效活性成分和209个有效炎症靶点;其核心活性成分包括槲皮素、芹菜素、熊果酸、β-谷甾醇、木犀草素、汉黄芩素等;关键靶点涉及PTGS2、PTGS1、CHRM1、DPP4、ADH1C等。GO功能和KEGG分析显示,与银黄制剂治疗肺及呼吸道炎症作用相关的生物过程(BP)条目339个,细胞组成(CC)条目100个,分子功能(MF)条目102个;KEGG相关信号通路225条(P<0.05),其中乙型肝炎、丙型肝炎、P13K/Akt信号通路、TNF信号通路、IL-17信号通路等与炎症密切相关。结论:本研究揭示了银黄制剂通过多活性成分、多靶点调节多通路来发挥对肺及呼吸道炎症的治疗作用,为进一步研究银黄制剂治疗肺及呼吸道炎症等外感性疾病的作用机制提供了参考依据和研究思路。Objective To analyze the mechanism of Yinhuang preparations in the treatment of exogenous disease by Network pharmacology.Methods The active components and targets of Yinhuang preparations were obtained by the TCMSP database,and the targets of pneumonia and respiratory tract inflammation including Pharyngitis,tonsillitis,bronchitis,pneumonia were retrieved by GeneCards,OMIM,and TTD database.Then the intersection targets of drugs and diseases were introduced into the STRING platform to construct protein-protein interaction(PPI)network,and then Cytoscape 3.6.1 software was used to construct the Yinhuang preparations-component–target network.Finally,gene ontology(GO)enrichment and KEGG pathway enrichment analysis were performed through the Metascape database to predict its mechanism.Results 222 active components of Yinhuang preparations were selected by database analysis.209 targets were screened out,and the core active ingredients of Yinhuang preparations for treating pneumonia and respiratory tract inflammation are quercetin,apigenin,ursolic acid,beta-sitosterol,luteolin,wogonin,etc.The core targets are PTGS2,PTGS1,CHRM1,DPP4,ADH1C,etc.GO functional enrichment analysis showed that there are 339 biological processes,100 cell composition,and 102 molecular functions.A total of 225 signal pathways(P<0.05)were screened by KEGG pathway enrichment analysis,which was mainly related to Pathways in Hepatitis B,Hepatitis C,PI3K-Akt signaling pathway,TNF signaling pathway,IL-17 signaling pathway and so on.Conclusion This study revealed the therapeutic effect of Yinhuang preparation on pneumonia and respiratory tract inflammation through multiple active components,multiple targets and multiple pathways.The results provide a reference basis and research ideas for further explaining the anti-pneumonia and anti-respiratory tractin flammation mechanism of Yinhuang preparation.

关 键 词：银黄制剂 外感性疾病 肺炎 网络药理学 作用机制 

分 类 号：TQ[化学工程]