一株具抗菌活性翡翠贻贝耐热真菌的鉴定及生理特性的研究  

Identification and Physiological Characteristics of Thermotolerant Fungus from Perna viridis with Antibacterial Activity

作　　者：杨永星 陈惠莹 顾哲铭 雷晓凌 聂芳红 Yang Yongxing;Chen Huiying;Gu Zheming;Lei Xiaoling;Nie Fanghong(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Provincial Engineering Technology Research Center of Seafood,Guangdong Province Engineering Laboratory for Marine Biological Products,Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东湛江524088 [2]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

出　　处：《广东化工》2022年第24期70-73,24,共5页Guangdong Chemical Industry

基　　金：广东省大学生创新创业训练计划项目(CXXL2018064)。

摘　　要：通过耐热试验以及牛津杯法从翡翠贻贝中分离筛选出具有良好抗菌活性的耐热海洋真菌PV-1。结合形态学与ITS测序确定菌株PV-1种属,采用单因素法进行培养基优化提升抗菌效果,用高效液相色谱法初步分析活性成分,通过测量菌落直径确定生理特性。PV-1经鉴定为土曲霉。实验表明,菌株PV-1对大肠杆菌(Escherichia coli)与吉氏库特氏菌SL-2(Kurthia gibsonii strain sp.)有良好的抗菌活性,经优化抑菌率分别提升至71.79%、71.52%,具有较强的抗菌活性,且富含极性与非极性活性成分;最适生长温度为35℃左右、盐度为1.5%~2.5%、pH值为6~7,具有兼性海洋特性,能在15~50℃、盐度0%~20%、pH值2~12条件下生长,具有极强的耐热、耐盐、耐酸碱能力,对环境耐受能力强,具有深入研究的价值。Thermotolerant marine fungi with PV-1 good antibacterial activity were isolated and screened from Perna viridis by heat-resistant test and Oxford cup method. The species of the preferred strain PV-1 was determined by combining morphology and ITS sequencing. The single factor method was used to optimize the culture medium to improve the antibacterial effect, and the active components were preliminarily analyzed by high performance liquid chromatography, and the physiological characteristics were determined by the determination of colony diameter. After identification, PV-1 is Aspergillus terreus. Experiments show that strain PV-1 has good antibacterial activity against Escherichia coli and Kurthia gibsonii strain sp. to 71.79 % and 71.52 %, has strong antibacterial activity, and is rich in polar and non-polar active ingredients;the optimum growth temperature is about 35 ℃, salinity is 1.5 %~2.5 %, pH value is 6~7. It can grow under the conditions of 15~50 ℃,salinity 0 %~20 %, pH value 2~12, and has strong heat resistance, salt resistance, acid and alkali resistance and facultative marine characteristics. It has strong environmental tolerance and has the value of in-depth research.

关 键 词：抗菌活性 翡翠贻贝 耐热真菌 鉴定 生理特性 

分 类 号：TQ[化学工程]