中药丹参水溶性成分丹酚酸B醇沉工艺优化研究  

Study on Alcohol Precipitation Process Optimization of the Water-Soluble Component of Salvia Miltiorrhiza Bunge, Salvianolic Acid B

作　　者：曾发古 苏倩 Zeng Fagu;Su Qian(School of Pharmacy,Guangdong Medical University,Dongguan 523808;Department of Pharmacy,The Seventh Affiliated Hospital of Sun Yat-sen University,Shenzhen 518107,China)

机构地区：[1]广东医科大学药学院,广东东莞523808 [2]中山大学附属第七医院药学部,广东深圳518107

出　　处：《广东化工》2022年第23期54-56,共3页Guangdong Chemical Industry

基　　金：广东医科大学校级项目(No:GDMUM2019015,GDMUM2020018);省级大学生创新创业训练计划项目(No:S202010571098);校级大学生创新创业计划项目(No:GDMU2019098,GDMU2020138)。

摘　　要：目的:优化丹参水溶性成分丹酚酸B的醇沉工艺方法:以溶液中总固体率、丹酚酸B的损失率以及丹酚酸B的含量作为指标,醇沉的体积分数、相对密度、温度、时间为影响因素,优化丹酚酸B的醇沉工艺结果:最佳条件为减压浓缩至相对密度1.05~1.15,边搅拌边加入95%乙醇,调乙醇含量至70%,在10~35℃环境下静置24 h,总固体去除率为27.3%,丹酚酸B的损失率4.96%,丹酚酸B的含量为13.81%。结论:该方法简单可行,可用于醇沉纯化丹参中丹酚酸B。Objective: To optimize the alcohol-precipitation process of salvianolic acid B, a water-soluble component of salvianolic acid from Salvia miltiorrhiza Bunge: Methods: Taking the total solid rate, the loss rate of salvianolic acid B and the content of salvianolic acid B as indexes, and the volume fraction, relative density, temperature and time as influencing factors, the alcohol precipitation process of salvianolic acid B was optimized. Results: The optimal conditions were as follows: concentration under reduced pressure to relative density of 1.05~1.15, adding 95 % ethanol while stirring, the ethanol content was adjusted to 70 %, standing at 10~35 ℃ for 24 h, the total solid removal rate was 27.3 %, and the loss rate of salvianolic acid B was 4.96 %. Conclusion: The method is simple and feasible, and it can be used to purify salvianolic acid B from Salvia miltiorrhiza Bunge by alcohol precipitation.

关 键 词：丹酚酸B 醇沉 工艺研究 水溶性成分 影响因素 

分 类 号：R284.2[医药卫生—中药学]