IL-17A基因-197A/G位点顺式调控机制及其对IL-17表达的影响  

The Cis-regulatory Mechanism of IL-17A Gene-197A/G Site and its Effect on IL-17 Expression

作　　者：李继荣 王志坚 陶保菊 LI Ji-rong;WANG Zhi-jian;TAO Bao-ju(Department of Infectious Diseases,the Second Naval Hospital,Southern Theater Command,Sanya 572000,Hainan,China)

机构地区：[1]南部战区海军第二医院感染科,海南三亚572000

出　　处：《医学信息》2023年第1期95-98,共4页Journal of Medical Information

摘　　要：目的探讨IL-17A基因-197A/G位点顺式调控作用及其对IL-17表达的影响。方法采用TaqMan系统对我院血液中心2021年2月1日-6月1日406名健康献血员全血样本进行IL-17A基因-197A/G位点基因分型,采用酶联免疫吸附试剂盒测定不同基因型个体血清IL-17水平,另通过电泳迁移率实验观察IL-17A基因-197A/G位点顺式调控作用。结果不同性别基因型分布及等位频率比较,差异无统计学意义(P>0.05);不同基因型个体间IL-17水平比较,差异有统计学意义(P<0.05),其中IL-17水平随基因型由AA[(296.12±63.43)pg/ml]→AG[(262.43±56.14)pg/ml]→GG[(159.17±31.46)pg/ml]变化呈递减趋势;电泳迁移率实验显示,一未知核因子与IL-17A基因-197A/G位点(rs2275913)A等位及G等位DNA探针特异性结合,但亲和力差异显著(A等位>G等位)。结论IL-17A基因-197A/G位点是一个非常关键的功能位点,通过特异性与一未知核转录因子结合而发挥对IL-17表达的顺式调控作用。Objective To investigate the cis-regulation mechanism of IL-17A gene-197A/G site and its effect on IL-17 expression.Methods The TaqMan system was used to genotype the IL-17A gene-197A/G locus in the whole blood samples of 406 healthy blood donors in the blood center of our hospital from February 1 to June 1,2021.The serum IL-17 levels of individuals with different genotypes were determined by enzyme-linked immunosorbent assay kit.In addition,the cis-regulation of IL-17A gene-197A/G locus was observed by electrophoretic mobility assay.Results There was no significant difference in genotype distribution and allele frequency between different genders(P>0.05).There were significant differences in IL-17 levels among different genotypes(P<0.05),while the level of IL-17 decreased with the change of genotype from AA[(296.12±63.43)pg/ml]→AG[(262.43±56.14)pg/ml]→GG[(159.17±31.46)pg/ml].Electrophoretic mobility shift assay showed that an unknown nuclear factor could specifically bind to A and G allelic DNA probes of IL-17A gene-197A/G site(rs2275913),but the affinity was significantly different(A allelic>G allelic).Conclusion The IL-17A gene-197A/G site is a very critical functional site,which plays a cis-regulatory role in IL-17 expression by specifically binding to an unknown nuclear transcription factor.

关 键 词：单核苷酸多态性 基因调控 顺式调控 

分 类 号：Q344.14[生物学—遗传学]