不同商品化病毒DNA提取试剂盒对非洲猪瘟病毒检测效果的比较研究  

Comparative study on the detection of African Swine Fever virus between the commercial kit for viral DNA extraction

作　　者：杨云龙 冯永智 高琦 吴小仪 龚浪 张桂红[1] YANG Yunlong;FENG Yongzhi;GAO Qi;WU Xiaoyi;GONG Lang;ZHANG Guihong(Guangdong Provincial Key Laboratory of Zoonoses Prevention and Control/College of Veterinary Medicine/South China Agricultural University/National Regional Laboratory of African Swine Fever(Guangzhou)/African Swine Fever Prevention and Control Technology Research Center/South China Agricultural University,Guangzhou Guangdong 510642)

机构地区：[1]华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室/国家非洲猪瘟区域实验室(广州)/华南农业大学非洲猪瘟防控技术研究中心,广东广州510642

出　　处：《广东畜牧兽医科技》2023年第1期39-43,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省重点领域研发计划(2019B020211003,2019B020211005);国家自然科学基金(31941004);财政部和农业农村部国家现代农业技术产业体系基金(CARS-35)。

摘　　要：为建立快速、准确的非洲猪瘟(African swine fever,ASF)检测方法,试验针对ASFV的晚期翻译p72蛋白基因构建了标准品质粒和标准曲线,并利用荧光定量PCR技术对OIE和CADC提供的引物和探针进行灵敏性的比对及对多种DNA提取试剂盒提取的ASFV DNA检测效率进行了评价,为ASFV的高效检测提供数据参考。结果表明:构建的ASFV晚期p72基因的标准品质粒和标准曲线中,p72标准品质粒的检测效率与灵敏度较优。通过使用OIE、CADC分别提供的引物、探针和多种DNA提取试剂盒进行ASFV荧光定量PCR检测,发现CADC提供的引物和探针对于ASFV的检测更加灵敏,Axygen品牌的DNA提取试剂盒对ASFV的检测性价比更高。In order to establish a rapid and accurate detection method for African swine fever(ASF),a standard plasmid and a standard curve were constructed for the late translation protein p72 gene of ASFV.The sensitivity of primers and probes provided by OIE and CADC was compared by fluorescence quantitative PCR technology,and the detection efficiency of ASFV DNA extracted by various DNA extraction kits was evaluated,providing data reference for the efficient detection of ASFV.The results showed that p72 standard plasmid had better detection efficiency and sensitivity among the standard plasmid and standard curve of p72 gene of ASFV constructed.Through the use of primers,probes and various DNA extraction kits provided by OIE and CADC for fluorescence quantitative PCR detection of ASFV,it was found that the primers and probes provided by CADC were more sensitive to the detection of ASFV,and the DNA extraction kit of Axygen brand was more cost-effective for the detection of ASFV.

关 键 词：非洲猪瘟病毒 p72 标准曲线 DNA提取试剂盒 

分 类 号：S852.65[农业科学—基础兽医学]