碱度长期胁迫对花鲈肾组织转录组的影响  

Kidney Transcriptomic Response of Lateolabrax maculatus to Long-Term Alkalinity Stressing

作　　者：王龙斌 泮淼军 王明阳 王润泽 李丽[1,2] 董双林 李卫东[3] 田相利 Wang Longbin;Pan Miaojun;Wang Mingyang;Wang Runze;Li Li;Dong Shuanglin;Li Weidong;Tian Xiangli(The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China;Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China;Tangshan Haidu Seafood Company Limited,Tangshan 063000,China)

机构地区：[1]海水养殖教育部重点实验室(中国海洋大学),山东青岛266003 [2]青岛海洋科学与技术试点国家实验室,山东青岛266237 [3]唐山海都水产食品有限公司,河北唐山063000

出　　处：《中国海洋大学学报（自然科学版）》2023年第2期32-43,共12页Periodical of Ocean University of China

基　　金：国家重点研究发展计划项目(2019YFD0900403);中国海洋大学-塔里木大学科研联合基金项目(ZHYLH201902)资助。

摘　　要：为了解花鲈(Lateolabrax maculatus)的耐碱机制,本研究探讨了碱度长期胁迫对花鲈肾组织转录组的影响。研究设计了3个碳酸盐碱度梯度(0、15和30 mmol/L),基于Illumina Novaseq 6000高通量测序平台进行了花鲈肾组织转录组测序并进行了生物信息学分析。研究表明,con组(0 mmol/L)、AW15组(15 mmol/L)和AW30组(30 mmol/L)分别获得了163197546、145296406和141892236条Clean reads。与con组相比,AW15组和AW30组分别有142和3103个差异表达基因上调,104和2235个差异表达基因下调。随机选取9条差异表达基因进行实时荧光定量PCR验证,结果与转录组测序一致,表明本研究中RNA-seq结果具有较高的可靠性。GO富集分析发现,差异基因显著富集在气体运输、GTP酶活性的正调控和水解酶活性的正调控等方面;KEGG通路富集分析表明,差异基因显著富集在细胞周期、DNA复制和癌症中的蛋白多糖等方面。本研究发现了49个持续变化基因(fyn、ceacam和acsl等),这些基因主要集中在机体生长、能量代谢、免疫反应等功能群中。中、高碱度长期胁迫对花鲈肾组织影响不同,其中,中碱度主要影响肾组织脂肪酸与氨基酸的合成与代谢,而高碱度则主要抑制了细胞有丝分裂和机体免疫能力。研究结果显示,花鲈对碱度的适应是一个涉及多基因的复杂过程,随着碱度升高,花鲈生长受到显著抑制,机体能量代谢增强,免疫能力显著下降。As a euryhaline marine teleost,spotted sea bass(Lateolabrax maculatus)has extraordinary ability to deal with high alkalinity(30 mmol/L).In order to effectively utilize saline-alkali water in southern Xinjiang and understand the tolerance mechanism of fish,we studied the effects of long-term alkalinity stressing on the kidney transcriptome of Lateolabrax maculatus.We employed Illumina Novaseq 6000 high throughput RNA sequencing to reveal the kidney transcriptomic difference among different carbonate alkalinities(0,15 and 30 mmol/L).The results showed that 163197546、145296406 and 141892236 Clean reads were obtained in control(0 mmol/L),AW15(15 mmol/L)and AW30(30 mmol/L)groups,respectively.Compared with the control,142 and 3103 differentially expressed genes were up regulated and 104 and 2235 differentially expressed genes were down regulated in AW15 and AW30 groups,respectively.Nine differentially expressed genes were randomly selected for real-time qPCR verification.The results were consistent with transcriptome sequencing.In GO enrichment analysis,we found that the differential genes were significantly enriched in gas transportation,positive regulation of GTPase activity and positive regulation of hydrolase activity.In KEGG pathway enrichment analysis,we found that the differential genes were enriched in phenylalanine,tyrosine and tryptophan biosynthesis,cell cycle and DNA replication.We found 49 continuously changing genes(fyn,ceacam,acsl among others),which involved in growth,energy metabolism and immunity.Overall,we found that medium and high alkalinities had different effects on kidney tissue of L.maculatus.Medium alkalinity mainly affected the synthesis and metabolism of fatty acids and amino acids while high alkalinity mainly affected mitosis and immune ability.In conclusion,with the increase of alkalinity,the growth of L.maculatus is inhibited,the energy metabolism is enhanced,the immune ability is decreased.The pathways and genes related to alkalinity stress in the kidney of L.maculatus obtained in this

关 键 词：碱度胁迫 花鲈 肾组织 GO富集 KEGG通路 响应基因 

分 类 号：S917.4[农业科学—水产科学]