氧化铁纳米颗粒对静止状态白血病细胞的作用研究  

The effects of iron oxide nanoparticles on quiescent leukemia cells

作　　者：李宛清 张雪 温涛 刘健[1] 孟洁[1] 许海燕[1] LI Wanqing;ZHANG Xue;WEN Tao;LIU Jian;MENG Jie;XU Haiyan(Institute of Basic Medical Sciences Chinese Academy of Medical Sciences,School of Basic Medicine Peking Union Medical College,Beijing 100005)

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础学院,北京100005

出　　处：《北京生物医学工程》2023年第1期52-56,60,共6页Beijing Biomedical Engineering

基　　金：国家重点研发计划项目(2017YFA02055504)资助

摘　　要：目的研究二巯基丁二酸修饰的四氧化三铁纳米颗粒(DMSA-Fe3O4NPs)对静止状态白血病细胞的作用及机制。方法利用透射电镜观察纳米颗粒形貌;用低血清培养基诱导细胞进入静止状态,采用普鲁士蓝染色法与邻二氮菲铁定量法检测DMSA-Fe3O4NPs进入Kasumi-1细胞的情况;通过乳酸脱氢酶法、细胞氧化应激荧光探针和凋亡检测试剂分析DMSA-Fe3O4NPs对Kasumi-1细胞的作用。结果DMSA-Fe3O4NPs可进入静止状态的Kasumi-1细胞,细胞内铁含量与DMSA-Fe3O4NPs浓度成正比;与此同时,细胞活性降低,细胞内活性氧(reactive oxygen species,ROS)与脂质过氧化物水平升高,细胞发生凋亡。正常培养的Kasumi-1细胞活性和氧化应激水平不受DMSA-Fe3O4NPs的影响。结论DMSA-Fe3O4NPs可进入静止状态的白血病细胞并引起细胞凋亡。Objective To investigate the effect of dimercaptosuccinic acid modified ferric oxide nanoparticles(DMSA⁃Fe3O4 NPs)on quiescent acute myeloid leukemia cell line(Kasumi-1).Methods The morphology of DMSA⁃Fe3O4 NPs was observed by transmission electron microscopy.The low serum containing medium was applied to induce Kasumi-1 cells to the quiescent state.The cellular uptake of DMSA⁃Fe3O4 NPs was analyzed by Prussian blue staining and phenanthroline spectrophotometric analysis.The release of lactate dehydrogenase,reactive oxidative species and apoptosis of Kasumi-1 treated with DMSA⁃Fe3O4 NPs at different concentrations were examined.Results The DMSA⁃Fe3O4 NPs entered the quiescent Kasumi-1 cells and the content of intracellular iron increased in a nanoparticles dose dependent manner.The viability of the quiescent Kasumi-1 cells was significant decreased and the intracellular reactive oxygen species(ROS)and lipid peroxide were increased as well.The Kasumi-1 cells cultured with the complete culture medium was not affected by DMSA⁃Fe3O4 NPs.Conclusions DMSA⁃Fe3O4 NPs could preferably enter the quiescent Kasumi-1 cells,which resulted in the upregulation of intracellular oxidative stress and cell apoptosis.

关 键 词：氧化铁纳米颗粒 白血病细胞 静止 氧化应激 凋亡 

分 类 号：R318.04[医药卫生—生物医学工程]