不同形态厌氧氨氧化污泥低温保存的性能差异研究  

Characterization of anammox biofilm and anammox granular sludge at low temperature preservation

作　　者：彭正华 杨本芹 杨志宏 詹友生 潘学军[1] PENG Zheng-hua;YANG Ben-qin;YANG Zhi-hong;ZHAN You-sheng;PAN Xue-jun(Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学环境科学与工程学院,云南昆明650500

出　　处：《中国环境科学》2023年第2期658-666,共9页China Environmental Science

基　　金：昆明理工大学高层次人才引进经费项目(10978191)。

摘　　要：厌氧氨氧化污泥的保存对其后续在反应器的脱氮过程有着重要的影响,因此,本研究针对不同形态的厌氧氨氧化污泥—生物膜和颗粒污泥的低温保存性能开展研究.结果表明,在64d 4℃的保存后,生物膜和颗粒污泥的厌氧氨氧化比活性分别降至(351.4±14.5),(32.3±2.7)mgN/(gVSS⋅d),分别为初始活性的62.1%和6.0%.EPS含量分别减少至(18.4±0.3)mg/gVSS和(13.3±1.5)mg/gVSS.生物膜和颗粒污泥中厌氧氨氧化功能菌属Candidatus Kuenenia丰度分别减少为6.1%和1.6%,厌氧氨氧化菌16S rRNA丰度分别降低为(1.48±0.29)×10^(8)gene copies/gVSS和(5.05±1.53)×10^(7)gene copies/gVSS.在后续的活性恢复过程中生物膜相较于颗粒污泥达到NRR为0.54kgN/m^(3)/d花费周期缩短了15d.因此,厌氧氨氧化生物膜是比颗粒污泥更好的保存形态.The preservation of anammox microorganisms is essential since it affects the subsequent reactor performance in nitrogen removal.The characters of anammox biofilm and anammox granular sludge at low temperature preservation were examined in this study.Results showed that after 64days preservation at 4℃,the specific anammox activities were found to be(351.4±14.5)mgN/(gVSS⋅d)for anammox biofilm and(32.3±2.7)mgN/(gVSS⋅d)for anammox granular sludge,which were 62.1%and 6.0%of those before preservation.Their EPS content decreased to(18.4±0.3)and(13.3±1.5)mg/gVSS,respectively.Moreover,the abundance of functional anammox genus,Candidatus Kuenenia,decreased from 16.3%to 6.1%in anammox biofilm and decreased from 16.8%to 1.6%in anammox granular sludge.Their anammox gene abundance also decreased to(1.48±0.29)×10^(8)and(5.05±1.53)×10^(7)gene copies/gVSS,respectively.During the reactivation of anammox process by using those two preserved anammox microorganisms,the time used to reach the NRR of 0.54kgN/m^(3)was lowered by 15days for anammox biofilm compared to that of anammox granular sludge showing the anammox biofilm was a better preservation form for anammox microorganisms than anammox granular sludge.

关 键 词：厌氧氨氧化 保存 细胞胞外聚合物(EPS) 微生物群落结构 

分 类 号：X703.5[环境科学与工程—环境工程]