蛋白质组学非靶向性质谱分析结合化学计量学在牛肉掺假初筛中的应用  

Non-targeted proteomics mass spectrometry combined with chemometrics for beef product preliminary screening

作　　者：蒲科源 丘嘉敏 刘柏霖 童永祺 程子彬 刘诚 林艳[4] 吴坤明 PU Keyuan;QIU Jiamin;LIU Bolin;TONG Yongqi;CHENG Zibin;LIU Cheng;LIN Yan;NG Kwanming(Department of Chemistry,Shantou University,Key Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province,Shantou 515063,China;Department of Biology,Shantou University,Shantou 515063,China;Department of Computer Science Shantou University,Shantou 515063,China;The Second Affiliated Hospital of Shantou University Medical College,Shantou 515041,China)

机构地区：[1]汕头大学化学系广东省有序结构材料的制备与应用重点实验室,广东汕头515063 [2]汕头大学生物系,广东汕头515063 [3]汕头大学计算机科学与技术系,广东汕头515063 [4]汕头大学医学院第二附属医院,广东汕头515041

出　　处：《食品与发酵工业》2023年第3期290-295,共6页Food and Fermentation Industries

基　　金：国家级大学生创新创业训练计划项目(202110560019)。

摘　　要：牛肉作为高价值食用肉类一直是肉制品造假的重灾区,构建一种高通量、快速、经济的牛肉鉴别方法势在必行。该研究在简单、快速地完成生、熟牛鸡鸭猪4种肉的蛋白质提取后,利用具有高通量、操作简便优势的基质辅助激光解析电离飞行时间质谱,采集蛋白质质谱数据,共获得生肉的129个离子峰和熟肉的151个离子峰,经数据预处理后,采用随机森林算法筛选到11个能区分4种肉的特征蛋白质,再结合主成分分析数据降维与数据可视化的优势,构建牛肉掺假鉴别模型,最后通过完成对掺假牛肉样本的检测以验证模型。结果表明,4种肉均能通过该方法得到较好的区分,且生熟状态下的掺假牛肉均能得到鉴别,证明该方法能准确、简便地完成生熟牛肉掺假初筛的目标。Beef,a kind of high-value edible meat,is always adulterated with low price meats in daily life.Therefore,the development of a reliable,high throughput,and economic method for beef identification is necessary.In this study,proteins of fresh and cooked chicken,duck,pork,and beef meats were extracted and then characterized with matrix-assisted laser desorption/ionization time of flight mass spectrometry.Totally,129 ion peaks of fresh meats and 151 ion peaks of cooked meats were obtained.Among them,11 characteristic proteins which enabled the differentiation of the 4 specifics of meat were discovered by random forest.Using the in-house prepared adulterated beef samples as the target samples,the capability of the 11 characteristic proteins for beef authentication was assessed with principal component analysis.Results showed that this method allowed the authentication of beef simply and reliably.

关 键 词：基质辅助激光解析电离飞行时间质谱 肉类掺假 机器学习 特征蛋白质 主成分分析 

分 类 号：TS251.52[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]