湖南省815株沙门菌血清型和耐药性分析  

Serotype and drug resistance of 815 Salmonella isolates in Hunan Province

作　　者：刘方 王岚[1] 湛志飞[1] 张林青[1] 袁青 陈帅[1] LIU Fang;WANG Lan;ZHAN Zhifei;ZHANG Linqing;YUAN Qing;CHEN Shuai(Department of Microbiology,Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410000,China)

机构地区：[1]湖南省疾病预防控制中心微生物检验科,湖南长沙410000

出　　处：《预防医学》2023年第2期134-136,140,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：科技部十三五科技重大专项课题(2018ZX10713003-002-008)。

摘　　要：目的 了解湖南省815株沙门菌的血清型和耐药性,为沙门菌感染防控提供参考。方法 对从2020—2021年湖南省食源性疾病腹泻患者粪便标本和食品标本分离出的沙门菌进行血清分型,采用微量肉汤稀释法进行药敏试验。结果815株沙门菌分离出10个血清群39种血清型,646株人源性菌株中B群388株,占60.06%;血清型以鼠伤寒及变种沙门菌为主,364株占56.35%;169株食源性菌株中B群61株,占36.09%;血清型以伦敦沙门菌为主,26株占15.38%。人源性耐药株597株,耐药率为92.41%,对氨苄西林耐药率较高,为81.58%;食源性耐药株140株,耐药率为82.84%,对四环素耐药率较高,为72.78%。两种来源的沙门菌对亚胺培南均敏感。多重耐药株577株,其中人源性多重耐药株490株,食源性多重耐药株87株。结论 2020—2021年湖南省815株从食源性疾病腹泻患者粪便标本和食品标本分离出的沙门菌血清型以鼠伤寒及变种沙门菌和伦敦沙门菌为主,多重耐药情况严重。Objective To investigate the serotype and drug resistance of 815 Salmonella isolates from Hunan Province,so as to provide insights into management of Salmonella infections. Methods Salmonella isolates were collected from stool samples of foodborne diarrheal patients and food samples in Hunan Province from 2020 to 2021, and serotyped.Antimicrobial susceptibility test was performed using the broth microdilution method. Results A total of 10 groups and39 serotypes were characterized in 815 Salmonella isolates. Among the 646 Salmonella isolates of human sources, 388isolates were identified as serogroup B(60.06%), with S. typhimurium and its variants aspredominant serotypes(364 isolates, 56.35%), and among 169 foodborne isolates, 61 isolates were characterized as serogroup B(36.09%) with S. london as the predominant serotype(26 isolates, 15.38%). There were 597 antimicrobial resistant Salmonella isolates of human sources, with a drug resistance rate of 92.41%, and the percentage of ampicillin resistance was 81.58%. There were 140 foodborne antimicrobial resistant isolates, with a drug resistance rate of 82.84%, and the proportion of tetracycline resistance was 72.78%. However, Salmonella isolates from both humans and foods were sensitive to imipenem. In addition, there were 577 multidrug resistant Salmonella isolates, including 490 multidrug resistant isolates of human sources and 87 foodborne multidrug resistant isolates. Conclusions S. typhimurium and its variants and S. london were predominant serotypes of Salmonella isolates from 815 foodborne diarrheal patients and food samples in Hunan Province from 2020 to 2021, and a high rate of multidrug resistance was detected.

关 键 词：沙门菌 血清型 耐药性 多重耐药 

分 类 号：R379[医药卫生—病原生物学]