幼鼠睾丸扭转对成年期健侧睾丸及性激素影响  

Influences of unilateral spermatic cord torsion in sex immature on histological changes of contralateral testis in sex mature rats and the detection of sex hormones levels in serum

作　　者：李刚龙 马洪 林金凤 陈进 张中严 赵鹏 刘红 Li Ganglong;Ma Hong;Lin Jinfeng;Chen Jin;Zhang Zhongyan;Zhao Peng;Liu Hong(Department of Pediatric Surgery,Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou,China;Department of General Surgery,Guangdong Women and Children Hospital,Guangzhou 510010,Guangdong,China)

机构地区：[1]遵义医科大学附属医院小儿外科,贵州遵义563003 [2]广东省妇幼保健院,广东广州510010

出　　处：《贵州医药》2023年第1期9-12,共4页Guizhou Medical Journal

基　　金：贵州省卫生厅科技项目,gzwkj2013-1-004;遵红科合社字(2017)17号。

摘　　要：目的了解鼠性成熟前期睾丸扭转后,对处于不同扭转状态的睾丸,采取何种手术方式,可最大限度地降低性成熟期健侧睾丸生殖功能的损害。方法采用Turner法制作鼠性成熟前期睾丸扭转模型,56只3周龄雄性SD鼠,随机分成7组、每组8只,任选一组行假手术,作为对照组(CG);余6组分别制作:扭转未坏死睾丸复位组(NNTR)、未坏死睾丸切除组(NNTO),扭转疑似坏死睾丸复位组(SNTR)、疑似坏死睾丸切除组(SNTO),以及扭转坏死睾丸复位组(NTR)、坏死睾丸切除组(NTO)。饲养6周至性成熟期、脱颈法处死大鼠,切取对侧睾丸石蜡包埋行HE染色检查组织结构的变化,留置心脏血液离心取血清、ELISA法检测性激素水平。结果性成熟期健侧睾丸组织结构改变:CG组健侧睾丸曲细精管内生精上皮无脱落,各级生精细胞排列有序,无生精小管萎缩;NNTR和NNTO组健侧睾丸均未出现形态学改变;SNTR和SNTO组健侧睾丸生精上皮细胞层减少,少量生殖细胞脱落于管腔中;NTR和NTO组中,前者睾丸生精上皮细胞层数明显减少,部分生殖细胞脱落于管腔中,生殖细胞排列紊乱,可见生精小管萎缩,后者未出现明显形态学改变。血清性激素(FSH、LH、T)水平:FSH、LH在各组中升高,T水平降低,但各性激素水平(FSH、LH、T)在NNTR与NNTO组间比较、以及SNTR与SNTO组间比较,差异均无统计学意义(P>0.05);性激素水平(LH、FSH、T)在NTR与NTO组间比较,NTR组的LH、FSH显著升高,T明显降低(P<0.05)。CG组各性激素水平(FSH、LH、T)分别与NNTR、NNTO以及NTO组间比较,差异均无统计学意义(P>0.05);而SNTR、SNTO以及NTR组FSH、LH均升高,T均降低(P<0.05)。结论性成熟前期扭转疑似坏死睾丸具有保留价值。Objective To find out what kind of operation that reservation or orchiectomy with different states of testicular torsion especially the suspected necrotic testis in sex immature rats can be adopted to reduce maximally the damage of reproductive function of contralateral testis in sex mature rats Methods The model of testicular torsion was made by Turner method.Fifty six male SD rats with 3-week-old were randomly divided into 7 groups with 8 rats in each group.One of them was done sham operation group and served as the control group(CG).The other group was made torsion Non-Necrotic Testis Reservation group/Orchiectomy group(NNTR/NNTO)and torsion Suspected Necrotic Testis Reservation group/Orchiectomy group(SNTR/SNTO)and torsion Necrotic Testis Reservation group/Orchiectomy group(NTR/NTO).After raising 6 weeks to sexual maturity,the rats were killed by decapitation.The contralateral testis was cut and embedded in paraffin for HE staining.At the same time,the serum was collected by centrifugation and the levels of sex hormones that Follicle stimulating Hormone(FSH),Luteinizing Hormone(LH)and Testosterone(T)were detected by ELISA.Results The histological changes of the contralateral testis in sexual maturity were as follows.The spermatogenic epithelium of the contralateral testis in CG did not fall off,the spermatogenic cells arranged orderly,and there was no atrophy of the seminiferous tubules.And so was also in NNTR and NNTO.The spermatogenic epithelial layer of contralateral testis decreased,and germ cells fell out of seminiferous tubules in SNTR and SNTO.The number of layers of spermatogenic epithelial cells was significantly reduced,the germ cells arranged disorderly and fell out of the lumen,and the seminiferous tubules atrophied in NTR while there was not obviously morphological changes in NTO.The levels of serum FSH and LH were increased and serum T was decreased in each group but there were no statistical significantly differences in the levels of sex hormones(FSH,LH,T)between NNTR and NNTO,and also between

关 键 词：鼠 睾丸扭转 形态学 卵泡刺激素 黄体生成素 睾酮 

分 类 号：R697.22[医药卫生—泌尿科学]