基于HPLC指纹图谱和化学模式识别的紫菀炮制前后对比研究  

Comparative study of Asteris Radix et Rhizoma before and after processing based on HPLC fingerprints combined with chemical pattern recognition

作　　者：韦飞扬 杜伟锋[2,3] 吴杭莎 吕悦 葛卫红 李昌煜[1] 张叶峰[4] 张光霁 WEI Fei-yang;DU Wei-feng;WU Hang-sha;LÜYue;GE Wei-hong;LI Chang-yu;ZHANG Ye-feng;ZHANG Guang-ji(College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 311402,China;Research Center of Traditional Chinese Medicine(TCM)Processing Technology,Zhejiang Chinese Medical University,Hangzhou 311401,China;Chinese Herbal Pieces Co.,Ltd.,Zhejiang Chinese Medical University,Hangzhou 311401,China;Ningbo Chinese Herbal Pieces Co.,Ltd.,Ningbo 315016,China;College of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China)

机构地区：[1]浙江中医药大学药学院,杭州311402 [2]浙江中医药大学中药炮制技术研究中心,杭州311401 [3]浙江中医药大学中药饮片有限公司,杭州311401 [4]宁波中药饮片有限公司,宁波315016 [5]浙江中医药大学基础医学院,杭州311402

出　　处：《药物分析杂志》2022年第11期2010-2018,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：宁波市“科技创新2025”重大专项(20201ZDYF020069);国家重点研发计划—中药饮片质量识别关键技术研究(2018YFC1707001)。

摘　　要：目的:建立紫菀生品和蜜炙品的HPLC指纹图谱,结合化学模式识别,分析判别生品紫菀和蜜紫菀,寻找紫菀炮制前后质量差异标志物,为紫菀炮制机理提供科学参考。方法:运用HPLC方法,Agilent Eclipse Plus C18色谱柱(250 mm×4.6 mm,5μm),流动相为0.1%磷酸水溶液-乙腈梯度洗脱,检测波长为200 nm,采用化学模式识别中的聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对紫菀炮制前后进行差异分析。结果:建立了紫菀生品和蜜炙品的HPLC指纹图谱,共有23个共有峰,经与对照品比对,指认出6个色谱峰,分别为绿原酸(5号峰)、异绿原酸B(8号峰)、异绿原酸A(9号峰)、槲皮素(16号峰)、山柰酚(19号峰)、紫菀酮(25号峰)。通过HCA、PCA、OPLS-DA的化学模式识别方法可将紫菀生品和蜜炙品分为2类,槲皮素、山柰酚、紫菀酮、异绿原酸等10个成分可能是紫菀炮制前后的质量差异标志物。结论:建立的HPLC指纹图谱结合化学模式识别分析的方法准确可靠,重复性好,可用于紫菀炮制前后的质量差异分析。Objective:To establish HPLC fingerprints of raw and honey processed Asteris Radix et Rhizoma,combined with chemical pattern recognition methods,to compare the differences of raw and honey processed Asteris Radix et Rhizoma,and to find the quality difference markers of Asteris Radix et Rhizoma before and after processing,providing scientific reference for the processing mechanism of Asteris Radix et Rhizoma.Methods:An HPLCmethod was established on an Agilent Eclipse Plus C18column(250 mm×4.6 mm,5μm)with acetonitrile-0.1%phosphoric acid as the mobile phase for gradient elution.The detection wavelength was 200 nm.The discriminant analysis of hierarchical cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were employed to distinguish the differences before and after the processing of Asteris Radix et Rhizoma.Results:The fingerprints of raw and honey processed Asteris Radix et Rhizoma were established and a total of 23 common peaks were marked.By comparison with the standards,six ingredients were identified.They were chlorogenic acid(F5),isochlorogenic acid B(F8),isochlorogenic acidA(F9),quercetin(F16),kaempferol(F19)and shionone(F25).The results of chemical pattern recognitiondemonstrated that raw and honey processed Asteris Radix et Rhizoma were divided into two categories.Ten components containing quercetin,kaempferol,shionone and isochlorogenic acid A might be the quality difference markers of them.Conclusion:The established fingerprint with chemical pattern recognition method is accurate,repeatable and reliable to apply in analyzing the quality differences before and after the processing of Asteris Radix et Rhizoma.

关 键 词：紫菀 蜜炙 指纹图谱 HPLC 槲皮素 紫菀酮 山柰酚 化学模式识别 质量评价 

分 类 号：R917[医药卫生—药物分析学]