Quick TargSeq全集成DNA快速检测系统在非实验室环境下对STR和DIP的同步检测分析  

Quick targseq rapid DNA integrated system applying to on-field multiplex detection of both STR and DIP loci

作　　者：万群 孙立霈 王寒 谢何鑫 孙文平 庄斌 韩俊萍 Wan Qun;Sun Lipei;Wang Han;Xie Hexin;Sun Wenping;Zhuang Bin;Han Junping(Shandong First Medical University,Jinan 250000,China;Key Laboratory of Forensic Genetics,Beijing Engineering Rearch Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science,Beijing 100038,China;Beijing CapitalBio Technology Ltd.Co.,Beijing 10llll,China;Technology Department of Chaoyang Sub-bureau,Beijing Public Security Bureau,Beijing 100025,China)

机构地区：[1]山东第一医科大学,山东济南250000 [2]公安部鉴定中心,法医遗传学公安部重点实验室,北京市现场物证检验工程技术研究中心,现场物证溯源技术国家工程实验室,北京100038 [3]北京博奥晶典生物技术有限公司,北京101111 [4]北京市公安局朝阳分局刑事侦查支队,北京100025

出　　处：《中国法医学杂志》2022年第6期538-541,546,共5页Chinese Journal of Forensic Medicine

基　　金：公安部“双十计划”重点推广项目(2020SSTG0504);2021年北京市现场物证检验工程技术研究中心开放课题项目(2021CSEEKFKT06)。

摘　　要：目的 测试Quick TargSeq全集成DNA快速检测系统在非实验室环境下对常见样本进行STR(Short Tandem Repeat)和DIP(Deletion-Insertion Polymorphisms)两种复合扩增试剂的检测能力。方法 将Quick TargSeq全集成DNA快速检测系统置于户外、车载等非实验室环境条件下,对59份样本(包含30份口腔拭子、26份血卡、2份接触类检材和1份精斑)进行STR检测,对43份样本(包含23份口腔拭子和20份血卡)进行DIP检测,对37份样本(20份口腔拭子和17份血卡)进行STR和DIP并行检测。结果 口腔拭子和血卡的位点检出率分别为98.95%和97.15%,全集成成功率均为100%,自动分型准确率分别为95.99%和94.81%,STR和DIP并行检测的检出率分别达到98.55%和97.96%,自动分型准确率分别为94.18%和98.82%;精斑和手机屏幕拭子可得到完整分型,而摩托车脚踏板拭子出现5个STR基因座丢失。结论 Quick TargSeq全集成DNA快速检测系统可以在非实验室环境下正常工作,具备STR和DIP两种复合扩增试剂的检测能力,且可以在同一卡盒的两个通道内实现两种试剂的并行检测,对于口腔拭子、血卡、精斑样本以及某些DNA含量高的接触类检材能够快速、自动获得分型信息。Objective To validate the performance of Quick TargSeq integrated rapid DNA analysis system to detect common samples with STR and DIP in a non-laboratory setting. Methods Quick TargSeq integrated rapid DNA analysis system was introduced in outdoor, vehicle and other non-laboratory settings. 59 samples(including 30 buccal swabs)、26 dried blood spot cards、2 contact samples and 1 seminal stain sample) were tested using the Quick TargSeq with premade STR reagent cartridges for individual identification. 43 samples(including 23 buccal swabs and 20 dried blood spot cards) were tested using pre-made DIP reagent cartridges. 37 samples(including 20 buccal swabs and 17 dried blood spot cards) were tested using cartridges contain both RTyper21 reagent and DIP reagent. Results The detection rates of buccal swabs and dried blood spot cards were 98.95% and 97.15 %, respectively. The success rates of them were both 100 %. The accuracy rates of automatic typing from buccal swabs and dried blood spot cards were 95.99% and 94.81 %, respectively. The detection rates of multipledetection for STR and DIP cartridges were 98.55 % and 97.96 %, respectively. The accuracy rates of automatic typing were 94.18 % and 98.82 %, respectively. The profile generated from both seminal sample and phone screen wipe were complete and concordant. The swab from a motorcycle pedal produced a partial STR profile with 5 alleles dropping out. Conclusion Quick TargSeq integrated rapid DNA analysis system can work in non-laboratory settings, with multiple-detection of both STR and DIP loci, and can detect different types of samples, such as buccal swab, dried blood spot card, seminal spot and touch DNA sample that contain high amounts of DNA in a fully-automated way.

关 键 词：法医遗传学 短串联重复序列 插入-缺失多态性 DNA现场快速检验 

分 类 号：D918.93[政治法律—法学]