磁性MOF固定化漆酶及其对刚果红降解效果研究  

Immobilized laccase on magnetic MOF for removal of Congo red

作　　者：李雪萍 王淋 陶喜洋 刘啸尘 武占省[1,2] LI Xueping;WANG Lin;TAO Xiyang;LIU Xiaochen;WU Zhansheng(School of Chemistry and Chemical Engineering,Shihezi University,Shihezi,Xinjiang 832003,China;School of Environmental and Chemical Engineering,Xi′an Polytechnic University,Xi′an,Shanxi 710048,China)

机构地区：[1]石河子大学化学化工学院,新疆石河子832003 [2]西安工程大学环境与化学工程学院,陕西西安710048

出　　处：《石河子大学学报（自然科学版）》2022年第6期669-676,共8页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(21868034)。

摘　　要：为克服游离漆酶稳定性差,重复利用性低的问题,本文通过共沉淀法制备以磁性纳米粒子(C@Fe_(3)O_(4))为核的铜基金属有机骨架材料(Cu-MOF),将漆酶(Laccase)包裹在Cu-MOF@C@Fe_(3)O_(4)中得到Lac@Cu-MOF@C@Fe_(3)O_(4)复合材料,并用于降解刚果红(CR)。通过XRD、FTIR和TGA等手段对材料进行表征,考察了酶固定化条件对固定化酶活性的影响。结果表明,Laccase成功固定在Cu-MOF@C@Fe_(3)O_(4)上;最优的固定化条件为:固定化温度30℃、固定化时间30 min、酶添加量3 mL、磁性纳米粒子的添加量30 mg。此外,所得固定化Laccase的pH稳定性、热稳定性均优于游离Laccase;循环使用5次后,固定化Laccase的残余活性仍高于70%,具有较好的可重复利用性。再者,90 min内,固定化漆酶对的刚果红溶液降解率能达到85%以上,研究结果可为新型酶固定材料的开发及其在废水处理中的应用提供参考。In order to overcome the problems of poor stability and low reusability of free laccase, in this paper, copper-based metal-organic framework(Cu-MOF) with magnetic nanoparticles(C@Fe_(3)O_(4)) as cores were prepared by co-precipitation method.The laccase were wrapped in Cu-MOF@C@Fe_(3)O_(4)to obtain Lac@Cu-MOF@C@Fe_(3)O_(4)composite materials, which were used to degrade Congo red(CR).The materials were characterized by XRD,FTIR and TGA.The effects of immobilization conditions on the activity of immobilized enzyme during the immobilization process were investigated.The results showed that laccase were successfully immobilized on Cu-MOF@C@Fe_(3)O_(4).The optimal immobilization conditions were immobilization temperature 30 ℃,immobilization time 30 min, enzyme addition volume 3 mL,magnetic nanoparticles addition volume 30 mg.In addition, Lac@Cu-MOF@C@Fe_(3)O_(4)had good stability and reusability.The pH stability and thermal stability of immobilized laccase were better than free laccase.After 5 cycles of use, the residual activity of Lac@Cu-MOF@C@Fe_(3)O_(4)was still higher than 70%.Furthermore, the degradation efficiency of Lac@Cu-MOF@C@Fe_(3)O_(4)for Congo red solution for 90 min could reach more than 85%.The results could provide references for the development of new enzyme immobilization materials and their applications in wastewater treatment.

关 键 词：Cu-MOF Fe_(3)O_(4) 漆酶 刚果红 

分 类 号：X703.1[环境科学与工程—环境工程]