基于红外光谱法对不同连作茬次地黄土壤光谱特征分析  

Analysis of Spectral Characteristics of Soil Under Different Continuous Cropping of Rehmannia Glutinosa Based on Infrared Spectroscopy

作　　者：乔璐[1] 刘瑞娜 张瑞 赵博宇 韩盼盼 周春亚 张雨晴 董诚明[1] QIAO Lu;LIU Rui-na;ZHANG Rui;ZHAO Bo-yu;HAN Pan-pan;ZHOU Chun-ya;ZHANG Yu-qing;DONG Cheng-ming(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;School of Pharmacy,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences Peking Union Medical College,Beijing 100193,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]广州中医药大学药学院,广东广州510006 [3]江西中医药大学药学院,江西南昌330004 [4]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《光谱学与光谱分析》2023年第2期541-548,共8页Spectroscopy and Spectral Analysis

基　　金：国家重点研发计划项目(2017YFC1702800);河南省重大科技专项项目(171100310500);河南中医药大学博士科研基金项目(BSJJ2014-01);河南省科技攻关基金项目(222102310182)资助。

摘　　要：连作障碍是中药材地黄种植过程中最大的难点,每茬收获后须隔8~10年后方可再种植,但连作障碍产生的机理尚不明确。提出一种基于傅里叶红外光谱技术(FTIR-ATR)和二维红外相关光谱(2D-IR)分析方法。采集正茬1年(YA-one)、重茬1年(YA-two)、10年前种植过(YA-ten)、空白(CK)4种地黄土壤样品的光谱,进行Norris滤噪平滑+二阶导数、二维相关光谱分析处理并提取特征光谱,分析土壤中化合物的指纹图谱。结果显示:不同连作茬次地黄土壤的峰形基本相似,红外光谱主要特征吸收峰在3621,3439,2932,2860,2513,1798,1636,1433,1029,887,783,695,546和469 cm^(-1)附近。与CK相比,YA-two的吸收强度最大,这些吸收峰表征酚酸中羟基—OH、羰基C=O、C—O、苯环的取代吸收等官能团,说明在重茬的地黄中,酚酸类物质积累。在2925和2857 cm^(-1)表征是糖苷类中饱和亚甲基—CH 2的反对称伸缩振动和对称伸缩振动,YA-two的吸收强度最小,YA-ten的吸收强度最大。说明重茬土壤中,糖苷类成分含量下降。不同连作茬次地黄土壤在920~1750,1490~1710和1500~1548 cm^(-1)中二维红外相关光谱,自动峰和交叉峰的位置、个数、以及强弱都不相同,清晰地表征出官能团的差异。结果表明采用傅里叶红外光谱和二维红外相关光谱能够实现土壤中有机化合物的快速检测,为研究地黄连作障碍研究提供理论依据。Continuous cropping obstacle is the greatest difficulty in Rehmannia glutinosa planting.It must be replanted after 8~10 years.However,the mechanism of the continuous cropping obstacles is not clear yet.An analytical method based on Fourier transform infrared spectroscopy(FTIR-ATR)and Two-Dimensional Infrared Correlation Spectroscopy(2D-IR)is proposed.Collected from the main crop(YA-One),second crop(YA-Two),10-year-old interval(YA-Ten)and blank(CK)of the spectra of four kinds of Rehmannia glutinos soil samples.Norris noise filtering and smoothing,second derivative and two-dimensional correlation spectrum analysis were performed to extract characteristic spectra,analyzed the fingerprint of compounds in the soil.The results showed that the peaks of different continuous cropping soils were similar,and the main characteristic absorption peaks of spectra were around 3621,3439,2932,2860,2513,1798,1636,1433,1029,887,783,695,546 and 469 cm^(-1).Compared with CK,the absorption intensity of YA-Two is the strongest,and these absorption peaks mostly represent the functional groups such as hydroxy—OH,carbonyl C=O,C—O,and substituted absorption of the benzene ring in phenolic acids,indicating the accumulation of phenolic acids in Rehmannia glutinos in continuous cropping.The reverse stretching vibration and symmetric stretching vibration of saturated methylene CH 2 in the glycosides were observed At t 2925 and 2857 cm^(-1).The absorption intensity of Ya-Two was the smallest,and Ya-Ten was the largest.The results indicated that the glucoside components decreased in continuous cropping soil.The position,number and strength of automatic peak and cross peak were different in the two-dimensional infrared correlation spectra of the soil of Rehmannia glutinos in bands 920~1750,1490~1710 and 1500~1548 cm^(-1),which clearly showed the difference of functional groups.The results showed that the rapid detection of organic compounds in soil could be achieved by using Fourier transform infrared spectroscopy(FTIR)and two-dimensional infr

关 键 词：地黄土壤 连作障碍 衰减全反射傅里叶变换红外光谱 二维红外相关光谱 酚酸类 糖苷类 

分 类 号：S153.6[农业科学—土壤学]