十八叔胺修饰硅胶色谱固定相制备及分离性能研究  

Preparation and chromatographic properties of silica gel chromatographic stationary phase modified with N,N-dimethyloctadecan-1-amine

作　　者：车轩 刘德禄 乔晓强[2] CHE Xuan;LIU Delu;QIAO Xiaoqiang(Hebei Ecological Environmental Monitoring Center,Shijiazhuang 050000,China;College of Pharmacy,Hebei University,Baoding 071002,China)

机构地区：[1]河北省生态环境监测中心,河北石家庄050000 [2]河北大学药学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2023年第1期40-47,共8页Journal of Hebei University(Natural Science Edition)

基　　金：河北省百名优秀创新人才支持计划(SLRC2019016)。

摘　　要：以十八叔胺(DMOA)为功能单体,通过叔胺基团与环氧功能化的二氧化硅进行开环反应,制备十八叔胺极性嵌合的SiO_(2)-DMOA色谱固定相.傅里叶变换红外光谱和热重分析证明SiO_(2)-DMOA色谱固定相已成功制备.保留机制研究显示:填充SiO_(2)-DMOA色谱柱具有反相/亲水混合模式保留机制,其柱效可达81100 N/m,并可实现苯胺类、烷基苯类、酰胺类、苯衍生物、苯酚类和多环芳烃类物质的有效分离.进一步将其用于磷脂标准品的分离,可实现磷脂类别和磷脂种类的有效分离分析.将其应用于大豆卵磷脂和肺腺癌细胞中磷脂的分离分析,显示了良好的分离潜力.With N,N-dimethyloctadecan-1-amine(DMOA)as functional monomer,the polar embedded SiO_(2)-DMOA chromatographic stationary phase was prepared through ring-opening reaction.The successful fabrication of the SiO_(2)-DMOA stationary phase was characterized by thermogravimetric analysis and Fourier infrared spectroscopy.The retention mechanism research shows that the packed SiO_(2)-DMOA column indicates reversed-phase/hydrophilic mixed-mode retention mechanisms and the column efficiency can reach 81,100 N/m.The SiO_(2)-DMOA column can achieve high-efficiency separation of anilines,alkylbenzenes,amides,benzene derivatives,phenols and polycyclic aromatic hydrocarbons.The SiO_(2)-DMOA column was further applied to the separation of phospholipid standards.It can realize the effective separation and analysis of both phospholipid classes and phospholipid species.The SiO_(2)-DMOA column was further applied to the separation and analysis of soybean lecithin and phospholipids in lung adenocarcinoma cells,which also show a good separation potential.

关 键 词：十八叔胺 色谱固定相 混合模式 磷脂 

分 类 号：O657.7[理学—分析化学]