Preliminary investigation and detection based on loop-mediated isothermal amplification(LAMP)of phytoplasmas associated with diseases in B.napus L.  

作　　者：Yancheng Wen Shufen Zhang Junping He Dongfang Cai Jiacheng Zhu Jianping Wang Jinhua Cao Kun Hu Lei Zhao Dongguo Wang Yizi Liu 

机构地区：[1]Institute of Industrial Crops of Henan Academy of Agricultural Sciences,Zhengzhou,450002,China

出　　处：《Oil Crop Science》2022年第4期219-224,共6页中国油料作物学报(英文版）

基　　金：the financial support provided by project of Science and Technology Tackling Key Problems in Henan Province(Grant No.182102110430);the National Key Research and Development Program of China(Grant No.2016YFD0101300);by China Agricultural Research system(CARS-12).

摘　　要：In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.

关 键 词：B.napus L. Phytoplasma associated disease PATHOGEN DETECTION Loop-mediated isothermal amplification (LAMP) 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]