控水干旱及PEG模拟干旱胁迫对谷子生理生化指标和SiVamp7基因转录水平的影响  

The Physiology Effects and Changes of SiVamp7 Transcription Levels of Foxtail Millet Seedlings Under Water-limiting and PEG-Simulated Drought Stress

作　　者：张犇 郭悦 刘丽文 郝冬冬 王小霞 杨埔 张丽珍[1] ZHANG Ben;GUO Yue;LIU Li-Wen;HAO Dong-Dong;WANG Xiao-Xia;YANG Pu;ZHANG Li-Zhen(School of Life Science,Shanxi University,Taiyuan 030036,China;State Key Laboratory of Sustainable Dryland Agriculture,Shanxi Agricultural University,Taiyuan 030031,China)

机构地区：[1]山西大学生命科学学院,太原030006 [2]山西农业大学省部共建有机旱作农业国家重点实验室(筹),太原030006

出　　处：《中国生物化学与分子生物学报》2023年第1期96-107,共12页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重点研发计划(No.2020YFD1001401);山西省筹资金资助回国留学人员科研项目(No.2021-015);省部共建有机旱作农业国家重点实验室(筹)开放基金(No.YJHZKF2108)资助。

摘　　要：为探讨控水干旱胁迫与聚乙二醇(polyethylene glycol,PEG)胁迫模拟干旱对谷子的影响,以5个不同地区的谷子品种陇谷16、晋谷21、冀谷39、大同40和济谷22为材料,使用控水干旱或PEG6000溶液模拟干旱处理植株,对逆境中苗期谷子的生理生化指标变化,特别是活性氧(reactive oxygen species,ROS)相关指标变化进行比较。同时,在分子生物学水平,比较了两种胁迫下,晋谷21中SiVamp7家族基因转录水平变化。结果表明,2种胁迫下,抗旱性由弱到强分别为陇谷16、晋谷21、冀谷39、大同40、济谷22。干旱胁迫下,抗旱品种的叶绿素含量维持较高水平(P<0.05),干旱敏感品种陇谷16抗氧化酶活性较低(P<0.05),导致氧化损伤严重,影响生长。对晋谷21中SiVamp7基因的转录水平检测表明,该基因家族对2种不同胁迫的响应不同。进一步研究表明,SiVAMP7蛋白与SiKAT3钾离子通道存在选择性互作,提示胁迫下,SiVamp7基因表达水平变化可能通过对钾通道调控,进而参与影响谷子干旱胁迫耐受。以上结果表明,PEG模拟干旱胁迫与控水干旱胁迫对谷子生理水平及基因表达水平影响不同,不能简单替换。This study aimed to investigate the effects of water-limiting drought stress and polyethylene glycol(PEG)-simulated drought stress on Foxtail millet seedlings.Five cultivars(Longgu16,Jingu21,Jigu39,Datong40,and Jigu22) were used in this study.Under two drought stresses,the generation and clearing of reactive oxygen species(ROS) and the changes in physiological and biochemical indexes of these five cultivars were analyzed.The changes in transcription levels of SiVamp7 genes in Jingu21 under two drought stresses were also analyzed.We found that five cultivars of foxtail millets showed a similar trend of drought resistance under two drought stresses.The drought-resistant cultivars kept high chlorophyll levels(P<0.05),while the activity of antioxidant enzymes in drought-sensitive cultivars was low under two drought stresses(P<0.05),which led to growth inhibition.Under two drought stresses,the transcription levels of SiVamp7 genes in Jingu21 were different.Further studies showed a selective interaction between the SiVAMP7 protein and SiKAT3 potassium channel,suggesting that the changes in SiVamp7 gene expression levels under stress might be involved in regulating drought stress tolerance by affecting the regulation of the potassium channel.Our results suggested that the effects of water-limiting drought stress and PEG-simulated drought stress differed in Foxtail millet.

关 键 词：谷子 控水干旱胁迫 聚乙二醇(PEG)胁迫 SiVamp7 

分 类 号：Q945[生物学—植物学]