丹参酮ⅡA抑制糖尿病动脉粥样硬化大鼠动脉平滑肌细胞凋亡及机制  

Tanshinone Ⅱ A inhibits the apoptosis of arterial smooth muscle cells in diabetic atherosclerotic rats and its mechanism

作　　者：符杰 邓红艳[1] 李玉凯 胡莹洁 胡嫚丽 FU Jie;DENG Hongyan;LI Yukai;HU Yingjie;HU Manli(Department of Endocrine,Wuhan Fourth Hospital,Wuhan,Hubei 430030,China)

机构地区：[1]武汉市第四医院内分泌科,湖北武汉430030

出　　处：《中南医学科学杂志》2023年第1期37-40,共4页Medical Science Journal of Central South China

基　　金：武汉市卫生健康科研基金资助项目(WZ19A06);湖北省卫生健康科研基金资助项目(WJ2019H413)。

摘　　要：目的 探讨丹参酮ⅡA对糖尿病动脉粥样硬化大鼠动脉平滑肌细胞凋亡的影响及机制。方法 将80只SPF级大鼠随机分为正常组、模型组、丹参酮ⅡA组和阿托伐他汀钙组,除正常组外,其余各组均采用腹腔内注射链脲佐菌素(STZ)及高糖高脂饲料喂养方法建立糖尿病动脉粥样硬化大鼠模型。观察各组大鼠动脉平滑肌细胞凋亡情况、血清超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平以及血管组织Toll样受体4(TLR4)及核因子-κB(NF-κB)mRNA表达。结果 与正常组相比,其他3组大鼠动脉平滑肌细胞凋亡、血清TNF-α、hs-CRP和IL-1β水平、TLR4及NF-κB mRNA表达明显升高(P<0.05)。与模型组相比,丹参酮ⅡA组和阿托伐他汀钙组大鼠的动脉平滑肌细胞凋亡、血清TNF-α、hs-CRP和IL-1β水平、TLR4及NF-κB mRNA表达降低(P<0.05),且丹参酮ⅡA组低于阿托伐他汀钙组(P<0.05)。结论 丹参酮ⅡA可降低糖尿病动脉粥样硬化模型大鼠动脉平滑肌细胞凋亡,降低炎症因子水平,其机制可能与抑制TLR4/NF-κB通路有关。Aim To investigate the effect of tanshinone ⅡA on the apoptosis of arterial smooth muscle cells in diabetic atherosclerosis rat model and its mechanism. Methods 80 SPF rats were randomly divided into normal group, model group, tanshinone Ⅱ A group and atorvastatin group, 20 rats in each group. The diabetic atherosclerosis rat model was made by intraperitoneal injection of streptozotocin(STZ) high glucose and high-fat diet. After 6 weeks, the apoptosis of arterial smooth muscle cells and serum high-sensitivity C-reactive protein(hs-CRP), tumor necrosis factor-α(TNF-α), and interleukin-1(IL-1) were observed. The expression of Toll-like receptor 4(TLR4) and nuclear factor-κB(NF-κB) mRNA in vascular tissue were detected. Results Compared with normal group, the arterial smooth muscle cell apoptosis and the levels of serum TNF-α and hs-CRP and IL-1β, TLR4 and NF-κB mRNA were significantly increased in the model group(P<0.05). Compared with the model group, the arterial smooth muscle cell apoptosis and the levels of serum TNF-α and hs-CRP and IL-1β, TLR4 and NF-κB mRNA were significantly decreasedintanshinone Ⅱ a group and atorvastatin group were significantly decreased(P<0.05). There were significant differences in the above indexes between the two groups(P<0.05). Conclusion Tanshinone Ⅱ A can inhibit the apoptosis of arterial smooth muscle cells and the level of inflammatory factors in diabetic atherosclerosis model rats, and its mechanism may be related to the inhibition of TLR4/NF-κB pathway.

关 键 词：丹参酮ⅡA 糖尿病 动脉粥样硬化 大鼠 平滑肌细胞 炎症因子 TLR4 NF-κB mRNA 

分 类 号：R587.1[医药卫生—内分泌]