一株鸡传染性喉气管炎病毒流行毒株的鉴定与致病性分析  

Preliminary Research and Virulence Assessment on One Virulent Strain of Infectious Laryngotracheitis Virus

作　　者：王静文[1] 张敏[1] 陈玲[1] 杨承槐[1] 蒋桃珍[1] WANG Jing-wen;ZHANG Min;CHEN Ling;YANG Cheng-huai;JIANG Tao-zhen(China Institute of Veterinary Drug Control,Beijing 100081,China)

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2023年第1期12-18,共7页Chinese Journal of Veterinary Drug

摘　　要：为评价一株鸡传染性喉气管炎病毒流行毒株在疫苗效力评价中的应用表现,对制备的一株强毒株进行了系统鉴定,包括性状、纯净性、真空度、病毒含量、特异性等,尤其重点关注不同病毒含量对不同日龄鸡只的致病力情况。结果显示,本研究制备的鸡传染性喉气管炎病毒冻干物性状良好,无细菌、支原体和外源病毒污染,可被特异性血清完全中和,病毒含量稳定在105.1EID50/0.2mL。该毒株致病力表现稳定,100 EID50可使80%鸡只发病或死亡,可作为一株良好的检验用强毒,为后续疫苗评价奠定基础。In order to evaluate the performance of a prevalent strain of infectious laryngotracheitis virus(ILTV)in the potency test for vaccine,a comprehensive identification of one freeze-drying virulent ILTV culture was conducted in terms of characteristics,purity,vacuum degree,viral loads,and specificity,with the focus on the assessment of virulence for chicken of various age post challenge.Results showed that this virulent strain has a good characteristic and purity,free from mycoplasma and exogenous virus contamination.It could be neutralized by specific serum and virus titers could reach 105.1EID50/0.2mL.At least 80%of unvaccinated control chicken died or showed severe clinical signs of ILT and notable macroscopic lesions at the dose of 100 EID50.Studies suggest that this prepared culture could be a good candidate as an appropriate challenge virus strain.

关 键 词：鸡传染性喉气管炎 疫苗 效力检验 检验用强毒 

分 类 号：S852.65[农业科学—基础兽医学]