盐酸多西环素片在猪体内的残留消除规律研究  

Residues Depletion Study of Doxycycline Hydroehloride Tablets in Pig

作　　者：赵富华[1] 陆连寿[1] 刘自扬[1] 李萌 李思奇 郭桂芳[1] 吴琴尧 曹玉颖 邱基程 李淑媛 张璐[2,3] 龚晓会 郭燕荧 张希墨 曹兴元 ZHAO Fu-hua;LU Lian-shou;Liu Zi-yang;LI Meng;LI Si-qi;GUO Gui-fang;WU Qin-Yao;CAO Yu-Ying;QIU Ji-cheng;LI Shu-yuan;ZHANG Lu;GONG Xiao-hui;GUO Yan-ying;ZHANG Xi-mo;CAO Xing-yuan(China Institute of Veterinary Drug Control,Beijing 100081,China;College of Veterinary Medicine China Agricultural University,Beijing 100093,China;Reference Laboratory for the test of Veterinary Drug Residues,Beijing 100093,China)

机构地区：[1]中国兽医药品监察所,北京100081 [2]中国农业大学动物医学院,北京100193 [3]国家兽药残留基准实验室,北京100193

出　　处：《中国兽药杂志》2022年第12期37-43,共7页Chinese Journal of Veterinary Drug

基　　金：动物源性细菌耐药性监测及兽药国家安全标准修订(16190158)。

摘　　要：研究盐酸多西环素片按说明书给药后在猪体内的残留消除规律并确定休药期。将多西环素片以5 mg/kg体重口服给药,间隔24 h,连续给药5次。最后一次给药之后分别在第0日(12 h)、1日、3日、6日和8日时间点采集猪的肌肉、肝脏、肾脏和脂肪,采用建立并验证的HPLC-VWD法测定猪不同组织中多西环素的含量。方法学考察结果表明,在0.05-5μg/mL添加范围内的线性方程和相关系数为Y=0.044x-0.414,R2=0.999。不同组织中的盐酸多西环素的平均回收率在60.32%~116.80%。为保证兽药使用安全、食品安全与人民健康,建议按照休药期计算结果确定盐酸多西环素片在猪体内的休药期为7日。In this study, it was determined the residual elimination rule of doxycycline hydrochloride tablets in pigs after administration according to the instructions and the drug withdrawal period. Doxycycline hydrochloride tablets were administered orally at 5 mg/kg of body weight, at an interval of 24 hours, for 5 consecutive doses. After the last administration, fat, muscle, liver and kidney were collected at 12 h, 1 d, 3 d, 6 d and 8 d respectively, and the content of doxycycline hydrochloride tablets in tissues was determined by HPLC-VWD. The linear range of doxycycline hydrochloride tablets in pig fat, muscle, liver and kidney tissues is 0.05-5 μg/ml. The linear equation and correlation coefficient are Y=0.044x-0.414, R~2=0.999. The average recovery rate of doxycycline hydrochloride in different tissues is 60.32%~116.80%. To ensure food safety and people’s health, it was suggested that the withdrawal time of doxycycline hydrochloride tablets in pig tissues should be no less than 7 days according to the calculation results.

关 键 词：多西环素 猪 残留消除 休药期 

分 类 号：S859.79[农业科学—临床兽医学]