人工加速老化法和低温发芽法的优化研究  

Research on Optimization of Artificial Accelerated Aging Test and Low Temperature Germination Test

作　　者：张齐齐 钱芳 谢磊 王戈 阿拉腾·巴根 蒋平安[4] 麻浩[1,2,4] ZHANG Qiqi;QIAN Fang;XIE Lei;WANG Ge;Alateng Bagen;JIANG Ping’an;MA Hao(State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing Jiangsu 210095,China;Inner Mongolia Academician Expert Workstation for Desert Ecological Industry,Alxa League Inner Mongolia 750306,China;Nanjing Lifecycle Environmental Protection Technology Company Limited,Nanjing Jiangsu 210049,China;Collaborative Innovation Center for Desert Ecological Restoration and Reconstruction,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]内蒙古荒漠生态产业院士专家工作站,内蒙古阿拉善盟750306 [3]南京三生万物环保科技有限公司,江苏南京210049 [4]新疆农业大学荒漠生态恢复与重建协同创新中心,乌鲁木齐830052

出　　处：《种子》2022年第12期1-6,共6页Seed

基　　金：国家自然科学基金项目(31760214)。

摘　　要：人工加速老化法和低温发芽法是测定种子活力的常用方法,但在荒漠植物上应用得很少。为了科学地将这两种方法应用在梭梭种子活力的测定上,需对其处理条件进行优化。本研究通过热水处理制备了4份不同活力水平的梭梭种子样品,比较不同老化温度(36℃、40℃、44℃)和老化时间(12 h、24 h、36 h、48 h、60 h、72 h、84 h、96 h)处理以及不同低温(10℃、8℃、6℃)处理对梭梭种子发芽的影响,对梭梭种子活力测定的人工加速老化法和低温发芽法进行了优化。结果表明,应用于梭梭种子活力测定的人工加速老化法的适宜条件为40℃老化36 h或48 h,低温发芽法的适宜条件为8℃或6℃。经优化后的人工加速老化法和低温发芽法都能够区分不同活力水平的梭梭种子样品,能较好地反映种子活力差异,可应用于梭梭种子活力的测定。Artificial accelerated aging test and low temperature germination test are common methods for determining seed vigor,but they are rarely used in desert plants.In order to scientifically apply them to the determination of Haloxylon ammodendron(C.A.Mey.)Bunge seed vigor,the treatment conditions should be optimized.In this study,four samples of H.ammodendron seeds with different vigor levels were firstly prepared by hot water treatment.On this basis,the artificial accelerated aging test and low temperature germination test for the determination of H.ammodendron seed vigor were optimized by comparing the effects of different aging temperature(36℃,40℃,44℃)and aging time(12 hours,24 hours,36 hours,48 hours,60 hours,72 hours,84 hours,96 hours)treatments and different low temperature(10℃,8℃,6℃)treatments on the germination indexes of H.ammodendron seed.The results showed that the suitable conditions for artificial accelerated aging test applied to the determination of H.ammodendron seed vigor were 40℃for 36 hours or 48 hours,and the suitable conditions for low temperature germination test were 8℃or 6℃.The optimized artificial accelerated aging test and low temperature germination test could distinguish H.ammodendron seed samples with different vigor levels and clearly reflect the difference in seed vigor,which could be well applied to the determination of H.ammodendron seed vigor.

关 键 词：梭梭 种子活力检测 人工加速老化法 低温发芽法 优化 

分 类 号：S604.1[农业科学—园艺学] Q[生物学]