亚精胺对盐胁迫下藜麦种子萌发和呼吸速率的影响  

Effects of Spermidine on Seed Germination and Respiration Rate of Chenopodium quinoa under Salt Stress

作　　者：卫丹丹 王丙全 赵丽娟[1] 张慧洁 张静瑶 WEI Dandan;WANG Bingquan;ZHAO Lijuan;ZHANG Huijie;ZHANG Jingyao(Xinzhou Normal University,Xinzhou Shanxi 034000,China;Maize Research Institute of Shanxi Agriculture University,Xinzhou Shanxi 034000,China)

机构地区：[1]忻州师范学院,山西忻州034000 [2]山西农业大学玉米研究所,山西忻州034000

出　　处：《种子》2022年第12期7-13,共7页Seed

基　　金：山西省高等学校科技创新项目(2020 L 0542);山西省基础研究计划(自由探索类)青年科学研究项目(20210302123366);忻州师范学院科研基金资助项目(2021 KY 12)。

摘　　要：以2个品种藜麦为试验材料,研究亚精胺(Spd)对盐胁迫下藜麦种子萌发的影响,分析Spd对NaCl胁迫下藜麦种子萌发率、淀粉含量和抗氧化酶活性等生理指标的影响。采用呼吸电子传递链专一性抑制剂水杨羟肟酸(SHAM)部分抑制交替氧化酶(AOX)呼吸途径,研究Spd和AOX途径与藜麦种子抗盐性的关系。结果表明,NaCl胁迫下,不同品种藜麦种子的最适Spd浓度不同;适宜浓度Spd提高藜麦种子的α-淀粉酶活性、促进淀粉分解,为藜麦种子萌发提供ATP;Spd通过提高CAT等抗氧化酶活性、提高呼吸速率,清除或减少氧自由基产生。外源施加适宜浓度Spd可以提高藜麦种子的抗盐性,提高盐胁迫下藜麦种子的呼吸速率,释放能量,促进藜麦种子萌发,而这种促进作用与AOX呼吸途径有关。In order to explore the effect of spermidine(Spd)on Chenopodium quinoa seed germination under salt stress,two Chenopodium quinoa varieties in this study were used as materials and the effect of Spd on physiological indexes such as germination rate,starch content and antioxidant enzyme activity of quinoa seeds under salt stress was analysed.Salicylhydroxamic acid(SHAM),a specific inhibitor of respiratory electron transport chain,was used to inhibit alternating oxidase(AOX)pathway partially to study correclation of Spd and AOX pathway to seed salt resistance.The results showed that the optimum Spd concentration of different varieties of quinoa seeds was different under NaCl stress.Improvement ofα-amylase activity by suitable concentration of Spd promoted starch decomposition and provided ATP for seed germination.Spd eliminated or reduced the production of reactive oxygen species by increasing the activity of antioxidant enzymes such as CAT increasing respiration rate.Exogenous application of appropriate concentration of Spd could improve salt resistance of quinoa seeds,improve respiration rate of quinoa seeds under salt stress,release energy and promote seed germination,and this promoting was related to AOX pathway.

关 键 词：亚精胺 盐胁迫 藜麦 种子萌发 呼吸速率 

分 类 号：S519[农业科学—作物学]