豹皮樟纤维素合酶基因家族的鉴定与表达分析  

Identification and Expression Analysis of Cellulose Synthase Gene Family in Litsea coreana var.sinensis

作　　者：姚新转 李倩倩 张宝会 吕立堂 YAO Xinzhuan;LI Qianqian;ZHANG Baohui;Lü Litang(College of Tea,Guizhou University,Guiyang 550025,China;Key Laboratory of Mountain Plant Resources Protection and Germplasm Conservation Innovation of Ministry of Education,Collaborative Innovation Center of Mountain Ecology and Agricultural Bioengineering,Institute of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学茶学院,贵阳550025 [2]贵州大学农业生物工程研究院/山地植物资源保护与保护种质创新教育部重点实验室/山地生态与农业生物工程协同创新中心,贵阳550025

出　　处：《种子》2022年第12期41-47,共7页Seed

基　　金：贵州省茶产业技术创新中心(黔科中引地[2017]4005);贵州省优秀青年科技人才培养项目(黔科合平台人才[2019]5651);贵州省科技计划项目(黔科合支撑[2021]一般111);CsHSF 24.1调控茶树儿茶素合成的分子机制解析(国家基金编号:32260789)。

摘　　要：纤维素合酶基因在初生细胞壁和次生细胞壁的合成中发挥着不同的作用,影响着豹皮樟纤维素含量以及老鹰茶的品质。本试验应用生物信息学方法,从豹皮樟中鉴定出17个纤维素合酶(CesA)基因,根据系统进化关系将这些基因分为7个亚组,进行了基因结构、染色体定位和不同部位基因表达分析。结果表明,所有基因均具有纤维素结构域,且不均等地分布在染色体上;RNA-seq数据分析表明,LcoCesA1_1/3_2/3_5/3_1/3_3/2_2在各个组织中表达较为显著,在根、茎以及其他部位中相对表达量普遍最高的是LcoCesA3_1,推测其在纤维素合成中发挥着重要作用。研究表明,该家族蛋白相对分子量为18834.17~128366 kD之间;理论等电点(pI)介于5.07~8.74之间,一半以上的LcoCesA蛋白富含酸性氨基酸。The cellulose synthase gene plays different roles in the synthesis of primary and secondary cell walls,affecting the cellulose content and quality of Litsea coreana var.sinensis.Using bioinformatics methods,17 CesAs were identified from Litsea coreana var.sinensis,and these genes were divided into 7 groups according to their phylogenetic relationship.Gene structure,chromosome localization,and gene expression analysis were conducted in different parts.The results showed that all genes had cellulose syn domains and were unevenly distributed on chromosomes;RNA-seq data analysis showed that LcoCesA1_1/3_2/3_5/3_1/3_3/2_2 was more prominently expressed in various tissues.LcoCesA3_1 had the highest relative expression in roots,stems and other parts,which was speculated to play an important role in cellulose synthesis.The study showed that the relative molecular weight of this family protein was from 18834.17 kD to 128366 kD;The theoretical isoelectric point(pI)was from 5.07 to 8.74,and more than half of the LcoCesA protein was rich in acidic amino acids.

关 键 词：纤维素 纤维素合酶 豹皮樟 表达分析 基因结构 

分 类 号：S792.23[农业科学—林木遗传育种]