外源甜菜碱对干旱胁迫下芫荽种子萌发及幼苗生理特性的影响  

Effects of Exogenous Glycine Betaine on Seed Germination and Physiological Characteristics of Coriandrum Sativum Seedlings under Drought Stress

作　　者：张二芳 李元圆 郝旺林 ZHANG Erfang;LI Yuanyuan;HAO Wanglin(Department of Life Sciences,Lüliang University,Lüliang Shanxi 033000,China;Institute of Soil and Water Conservation,Chinese Academy of Sciences and Ministry of Water Resources,Yangling Shannxi 712100,China;University of Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]吕梁学院生命科学系,山西吕梁033000 [2]中国科学院水利部水土保持研究所,陕西杨凌712100 [3]中国科学院大学,北京100190

出　　处：《种子》2022年第12期93-97,105,共6页Seed

基　　金：国家自然科学基金(41771318,41830758);山西省高等学校科技创新项目(2020 L 0680);吕梁市重点研发项目(2019 SHFZ 75)。

摘　　要：以芫荽种子为实验材料,采用9%聚乙二醇(PEG-6000)模拟干旱胁迫,研究了不同质量浓度(10、20、40、80 mg/L)的甜菜碱(GB)对芫荽种子萌发和幼苗生理指标的影响,以未加PEG和GB的蒸馏水组为空白对照(ck),以只添加PEG未添加GB的为条件对照(CH)。结果表明,与ck组相比,PEG模拟干旱胁迫下,芫荽种子的发芽率、发芽势、发芽指数、活力指数显著下降,幼苗叶绿素含量显著下降,过氧化物酶(POD)和过氧化氢酶(CAT)活性显著增高,丙二醛(MDA)、脯氨酸(Pro)和可溶性蛋白含量显著上升;与CH组相比,随外源GB浓度的增高,芫荽种子的发芽率、发芽势、发芽指数、活力指数先增高后降低,芫荽幼苗叶绿素含量、POD、CAT活性先增高后降低,MDA、Pro和可溶性蛋白含量先降低后升高,其中20 mg/L的GB对PEG胁迫下芫荽种子萌发和幼苗生长影响的缓解效果最显著,显著提高了芫荽的抗旱能力。The effects of different mass concentrations(10,20,40,80 mg/L)of glycine betaine(GB)on the germination and seedling physiological characteristics of Coriandrum sativum seeds were studied by using 9%polyethylene glycol(PEG-6000)to simulate drought stress,the blank control(ck)was treated with distilled water without PEG,the conditional control(CH)was treated with PEG without GB.The results showed that compared with ck group,the germination rate,germination potential,germination index and vitality index of coriander seeds significantly decreased,the chlorophyll content of seedlings significantly decreased,the activities of peroxidase(POD)and catalase(CAT)significantly increased,the contents of malondialdehyde(MDA),proline(Pro)and soluble protein increased significantly under PEG simulated drought stress.Compared with CH group,the germination percentage,germination potential,germination index and vitality index of coriander seeds increased firstly and then decreased with the increase of exogenous GB concentration,the contents of chlorophyll,POD and CAT in coriander seedlings increased firstly and then decreased,while the contents of MDA,Pro and soluble protein decreased firstly and then increased.The relaeased effect of 20 mg/L GB on the germination and seedling growth of coriander seeds was the most significant,significantly improved the drought resistance of coriander.

关 键 词：芫荽 甜菜碱 干旱胁迫 种子萌发 生理特性 

分 类 号：S636.9[农业科学—蔬菜学]