TTC法快速测定葛根种子生活力  

Quickly Determine the Viability of Pueraria lobata Seeds by TTC Method

作　　者：莫周美 张秀芬 李恒锐 郭素云 何文 黄珍玲 李天元 MO Zhoumei;ZHANG Xiufen;LI Hengrui;GUO Suyun;HE Wen;HUANG Zhenling;LI Tianyuan(Guangxi South Subtropical Agricultural Science Research Institute,Longzhou Guangxi 532415,China)

机构地区：[1]广西农业科学院/广西南亚热带农业科学研究所,广西龙州532415

出　　处：《种子》2022年第12期126-131,共6页Seed

基　　金：国家现代农业产业技术体系广西创新团队建设项目(nycytxgxcxtd-1-07);广西农业科学院基本科研业务专项(桂农科2022 YM 35、桂农科2021 YT 157)。

摘　　要：采用TTC法测定葛根种子生活力,通过单因素试验和Box-Benhnken响应面试验,探究染色时间、TTC浓度和染色温度对TTC染色的影响。结果表明,葛根种子TTC法染色的最佳条件为:染色时间2 h、TTC浓度0.57%、染色温度37.3℃,在优化条件下测得葛根种子平均生活力为84.44%,实际测定值与理论预测值误差为3.71%(<5%),说明响应面法优化葛根种子TTC法测定条件是可行的。The seed viability was determined by TTC method,and the effects of dyeing time,TTC concentration and dyeing temperature on TTC dyeing were investigated by single factor test and Box-Benhnken response surface test.The results showed that the optimal conditions for TTC dyeing of pueraria seeds were as follows:dyeing time for 2 hours,TTC concentration of 0.57%,dyeing temperature at 37.3℃,the average viability of pueraria seeds was measured to be 84.44%under the optimized conditions.The error of the predicted value was 3.71%(<5%),indicating that the response surface methodology was feasible to optimize the TTC determination conditions of pueraria seeds.

关 键 词：葛根 种子活力 TTC法 响应面法 

分 类 号：S567.19[农业科学—中草药栽培]