元宝枫、三角枫及金沙枫叶片柯里拉京含量的变异与季节变化  

Variation and Seasonal Difference of Corilagin Content in Leaves of Acer truncatum, Acer buergerianum and Acer paxii

作　　者：苏亚静 鲁仪增 乔谦 孙忠奎 武冲[3] 燕语 程甜甜[5] 张林[5] 丰震[1] SU Ya-jing;LU Yi-zeng;QIAO Qian;SUN Zhong-kui;WU Chong;YAN Yu;CHENG Tian-tian;ZHANG Lin;FENG Zhen(Shandong Agricultural University,Taian 271018,Shandong,China;Shandong Provincial Center of Forest and Grass Germplasm Resources,Ji’nan 250102,Shandong,China;Shandong Institute of Pomology,Taian 271000,Shandong,China;Tai'an Times Garden Technology Development Co.,Ltd,Taian 271000,Shandong,China;Taishan Forestry Science Institute,Taian 271000,Shandong,China)

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]山东省林草种质资源中心,山东济南250102 [3]山东省果树研究所,山东泰安271000 [4]泰安时代园林科技开发公司,山东泰安271000 [5]泰安市泰山林业科学研究院,山东泰安271000

出　　处：《林业科学研究》2023年第1期31-38,共8页Forest Research

基　　金：山东省农业良种工程项目:新型木本油料树种突破性新品种选育(2020LZGC009);中央财政林业改革发展资金项目:“元宝枫良种繁育及丰产栽培技术示范”([2020]TG01)和元宝枫种质资源收集评价及种质挖掘(2020KJCX01);泰安农业良种工程“元宝枫新品种定向选育及示范应用”。

摘　　要：[目的]为进一步开发元宝枫、三角枫和金沙枫的药用价值,丰富用于提取柯里拉京的植物资源并提升这3种枫树的药用价值以及筛选药赏兼用型品种。[方法]采用超高效液相色谱法(UPLC)对元宝枫、三角枫和金沙枫叶片中的柯里拉京进行测定,以XBridge C_(18)(4.6×150 mm,3.5μm)为色谱柱,以0.4%甲酸水溶液和甲醇为流动相,流速为0.3 mL·min^(-1),柱温为25℃,检测波长270 nm,进样量2μL。[结果]元宝枫、三角枫和金沙枫的叶片中都含有柯里拉京;不同树种之间柯里拉京含量存在较大差异,三角枫含量最高,金沙枫次之,元宝枫最低;不同品种之间柯里拉京含量也存在较大差异,三角枫4个品种中‘兴旺’平均含量最高,而‘千里红’在10月达到6.888 7 mg·g^(-1)的峰值;金沙枫‘鲁绿’在6月达最高值2.664 0 mg·g^(-1);元宝枫4个品种中,‘绿宝石’在6月达最高值0.607 9 mg·g^(-1),不同采收时间柯里拉京含量也存在较大差异,三角枫峰值多在10月,元宝枫和金沙枫最高值多出现在5月和6月。[结论]首次揭示元宝枫、三角枫和金沙枫叶片中含有柯里拉京,其中,三角枫更适合作为提取柯里拉京的原材料,金沙枫和元宝枫可作为候选资源。[Objective]To determine the content of corilagin in leaves of Acer truncatum Bunge,A.buergerianum Miq.and A.paxii Franch.[Methods]The ultra-performance liquid chromatography(UPLC) were used.XBridge C_(18)(4.6 × 150 mm,3.5 μm) was used as the chromatographic column,and 0.4% formic acid aqueous solution and methanol as the mobile phase with the flow rate of 0.3 mL·min^(-1),the column temperature of 25℃,the detection wavelength of 270 nm,and the injection volume of 2 μL.[Results]The leaves of A.truncatum Bunge,A.buergerianum Miq.and A.paxii Franch.contained corilagin.There were significant differences in the content of corilagin among different tree species.The content in A.buergerianum Miq.was the highest,followed by A.paxii Franch.and A.truncatum Bunge.There were also significant differences in the content of corilagin among different varities.Among the four varieties of A.truncatum Bunge,’Lvbaoshi’ reached the highest value of 0.607 9 mg·g^(-1),the average content in ’Xingwang’ among the four cultivars of A.buergerianum Miq.was the highest,and ’Qianlihong’ had the highest value(6.888 7 mg·g^(-1)) in October,and the highest valuein A.paxii Franch.’Lulv’ was 2.664 0 mg·g^(-1).In addition,there was also a big difference in the content of corilagin at different harvest times.The highest values of A.truncatum Bunge and A.paxii Franch.mostly appeared in May and June,and the best harvesting time of A.buergerianum Miq.was in October.[Conclusion]This is the first time for revealing that there is corilagin in the leaves of A.truncatum Bunge,A.buergerianum Miq.and A. paxii Franch.,among which A.buergerianum Miq.is more suitable as the raw material for extracting corilagin,and A. truncatum Bunge and A. paxii Franch.can be used as candidate resources.

关 键 词：柯里拉京 元宝枫 三角枫 金沙枫 超高效液相色谱法 

分 类 号：S718.43[农业科学—林学]