荧光染色法与KOH法在皮肤真菌直接镜检检查中的应用效果比较  

Comparison of the Effect of Fluorescence Staining and KOH Method in Direct Microscopic Examination of Skin Fungi

作　　者：曾莉 ZENG Li(Xinyang People's Hospital,Xinyang 464000,Henan Province,China)

机构地区：[1]信阳市人民医院,河南信阳464000

出　　处：《罕少疾病杂志》2023年第2期104-105,共2页Journal of Rare and Uncommon Diseases

摘　　要：目的比较荧光染色法与KOH法在皮肤真菌直接镜检检查中的应用效果。方法采集皮肤科门诊98例疑为皮肤真菌感染患者皮损标本,每例均进行荧光染色法与KOH法检测,比较检测结果。对比两种方法阳性率、不同标本阳性率、不同疾病类型阳性率。结果总98例标本中,荧光染色法阳性63例,阴性35例,阳性率为64.29%;KOH法阳性46例,阴性52例,阳性率为46.94%,两者差异有统计学意义(χ^(2)=5.973,P=0.015)。以KOH法为参考,荧光染色法相对灵敏度100%,相对特异度为67.31%;采用荧光染色法检测皮屑、甲屑、毛发阳性率均较KOH法高,荧光染色法阳性率87.76%,KOH法阳性率68.37%,差异有统计学意义(χ^(2)=10.755,P=0.001);98例患者中,皮屑标本87例,其中体股癣最多,其次为手足癣,甲真菌病的甲屑标本3例,头癣的毛发标本8例。荧光染色法阳性检出率较KOH法高(χ^(2)=18.092,P<0.001)。结论荧光染色法较KOH法结果更加准确、迅速,能便于快速诊断皮肤真菌感染状况。Objective To compare the effect of fluorescence staining and KOH method in direct microscopic examination of skin fungi.Methods A total of 98 patients with suspected fungal infection were collected from the outpatient department of dermatology.Fluorescence staining and KOH method were used to detect the lesions in each case.The positive rates of the two methods,different specimens and different disease types were compared.Results Of the 98 samples,63 were positive and 35 were negative by fluorescence staining,the positive rate was 64.29%.There were 46 positive cases and 52 negative cases by KOH method,the positive rate was 46.94%(χ^(2)=5.973,P=0.015).With KOH method as reference,the relative sensitivity and relative specificity of fluorescence staining were 100%and 67.31%.The positive rates of dandruff,nail dandruff and hair detected by fluorescence staining method were higher than that of KOH staining method.The positive rate was 87.76%by fluorescence staining and 68.37%by KOH staining,and the difference was statistically significant(χ^(2)=10.755,P=0.001);Of the 98 patients,87 were skin splatyskin specimens,psoriasis psoriasis was the most psoriasis,hand-foot psoriasis was the next,onychomycosis was 3 cases,psoriasis was 8 cases hair.The positive rate of fluorescence staining was higher than that of KOH staining(χ^(2)=18.092,P<0.001).Conclusion Fluorescence staining method is more accurate and rapid than KOH method,and it is convenient for rapid diagnosis of skin fungal infection.

关 键 词：皮肤真菌感染 荧光染色法 KOH法 不同疾病类型阳性率 

分 类 号：R756[医药卫生—皮肤病学与性病学]