小麦转录因子TaMYB5-3B与株高和千粒重相关  

Transcription factor TaMYB5-3B is associated with plant height and 1000-grain weight in wheat

作　　者：朱治 李龙[2] 李超男 毛新国[2] 郝晨阳[2] 朱婷 王景一[2] 常建忠 景蕊莲[2] ZHU Zhi;LI Long;LI Chao-Nan;MAO Xin-Guo;HAO Chen-Yang;ZHU Ting;WANG Jing-Yi;CHANG Jian-Zhong;JING Rui-Lian(Shanxi Institute of Organic Dryland Farming,Shanxi Key Laboratory of Organic Dry Farming,State Key Laboratory of Integrative Sustainable Dryland Agriculture(in Preparation),Shanxi Agricultural University,Taiyuan 030031,Shanxi,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]山西农业大学山西有机旱作农业研究院/有机旱作山西省重点实验室/省部共建有机旱作国家重点实验室(筹),山西太原030031 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2023年第4期906-916,共11页Acta Agronomica Sinica

基　　金：山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202105D121008-2-7);财政部和农业农村部国家现代农业产业技术体系建设专项(小麦,CARS-03)资助。

摘　　要：MYB转录因子在植物生长发育过程中发挥着重要作用。本研究克隆了小麦3B染色体上的TaMYB5-3B基因,基因组序列全长3005bp,其中编码区上游为2112bp,编码区为893bp,包含2个外显子和1个内含子,编码一个R2R3-MYB蛋白。序列多态性分析表明,在TaMYB5-3B的–2048、–1632、–1178、–1156、–504、–461、–433和61 bp处各有1个SNP位点,分别是G/A转换、G/A转换、G/A转换、T/C转换、C/T转换、A缺失、T缺失和T/A颠换,这8个SNP位点连锁。基于启动子区SNP-1632的变异开发分子标记,检测小麦自然群体的基因型,与表型性状进行关联分析,结果显示TaMYB5-3B与株高、穗下节长和千粒重显著相关。TaMYB5-3B在群体中有2种单倍型Hap-3B-1和Hap-3B-2,其中Hap-3B-2是植株较矮、千粒重较高的优异单倍型。在我国的小麦育种历程中Hap-3B-2受到了正向选择,在育成品种中的频率逐步增加,但仍然有进一步的应用潜力。研究结果为深入探讨小麦株高和产量的形成机制提供参考,也为小麦株型和产量分子育种提供了基因资源与选择标记。MYB transcription factor plays an important role in plant growth and development.In this study,we cloned TaMYB5-3B gene on chromosome 3B in wheat.The full-length genome sequence is 3005 bp,including 2112 bp promoter region and 893 bp coding region.TaMYB5-3B coding region consists of two exons and one intron,which encodes a R2R3-MYB protein.The polymorphism of TaMYB5-3B was analyzed by sequencing 32 wheat accessions with wide variations.A total of eight SNPs were detected at–2048,–1632,–1178,–1156,–504,–461,–433,and 61 bp,respectively.They were eight SNPs linked by G/A conversion,G/A conversion,G/A conversion,T/C conversion,C/T conversion,A deletion,T deletion and T/A inversion,respectively.A pair of molecular markers were developed based on the promoter region SNP-1632 to detect the genotypes of wheat natural population.The association analysis of genotype and phenotypic traits showed that TaMYB5-3B was significantly associated with plant height(PH),peduncle length(PLE),and 1000-grain weight(TGW).Two haplotypes(Hap-3B-1 and Hap-3B-2)were detected in the population,in which Hap-3B-2 was an excellent haplotype with short PH and high TGW.Hap-3B-2 had been positively selected in the breeding,and its frequency in modern cultivars gradually increased with the advance of breeding years in China.Therefore,Ta MYB5-3B could be used to further understand the mechanism of wheat plant height and grain yield formation,and its molecular markers may contribute to ideal plant architecture and grain yield breeding of wheat.

关 键 词：分子标记 株高 千粒重 单倍型 关联分析 小麦 

分 类 号：S512.1[农业科学—作物学]