木薯MYB转录因子基因MeMYB60表达特征分析及其互作蛋白筛选  

Expression pattern analysis and interaction protein screening of cassava MYB transcription factor MeMYB60

作　　者：徐子寅 于晓玲[2,3] 邹良平 赵平娟[2,3] 李文彬 耿梦婷[1] 阮孟斌 XU Zi-Yin;YU Xiao-Ling;ZOU Liang-Ping;ZHAO Ping-Juan;LI Wen-Bin;GENG Meng-Ting;RUAN Meng-Bin(College of Tropical Crops,Hainan University,Haikou 570228,Hainan,China;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China;Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province,Hainan Institute for Tropical Agricultural Resources,Haikou 571101,Hainan,China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [3]海南热带农业资源研究院/海南省热带农业生物资源保护与利用重点实验室,海南海口571101

出　　处：《作物学报》2023年第4期955-965,共11页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2018YFD1000501);海南省重大科技计划项目(ZDKJ2021012);中央级公益科研院所基本科研业务费专项(1630052022036)资助。

摘　　要：Myeloblastosis(MYB)类转录因子在植物对非生物胁迫的响应过程中起重要调控作用。本研究在分析栽培木薯MYB家族成员表达模式的基础上,筛选并克隆到了一个R2R3-MYB转录因子基因MeMYB60。基因表达特性分析表明,该基因在叶片特异表达,受干旱、低温负调控,同时对ABA处理也有响应。启动子活性分析发现,MeMYB60可以在保卫细胞表达,预示着该转录因子基因的表达可能与木薯气孔开闭调节有关。MeMYB60编码蛋白主要定位于细胞核中,具有转录激活活性,其转录激活结构域在蛋白C端第194~343氨基酸残基范围内。以MeMYB60蛋白N端第1~194氨基酸残基片段为诱饵,从干旱胁迫后木薯叶片的cDNA文库中筛选到18种可能与MeMYB60互作的蛋白,酵母双杂确定了MeCatlase1和MeCatalase2分别与MeMYB60存在互作关系。本研究为深入研究转录因子MeMYB60在木薯响应非生物胁迫过程中的功能并解析其调控网络奠定了基础。Myeloblastosis(MYB)transcription factors widely involve in a variety of physiological and biochemical processes in plants,and play important regulatory roles in response to abiotic stress in plant.Based on the expression pattern of MYB members in cassava cultivars,an R2R3-MYB transcription factor,namely MeMYB60 was screened and cloned.Gene expression characteristics showed that MeMYB60 was specifically expressed in leaves of cassava,and negatively regulated by drought stress and low temperature.Moreover,this gene was also responded to ABA treatment in leaves of cassava.Promoter activity analysis showed that MeMYB60 could be expressed in guard cells,indicating that the expression of this transcription factor gene may be related to stomatal movement regulation in cassava.MeMYB60 protein was predominately located in the nucleus and had transcriptional activation activity.Its transcriptional activation domain was in the range of 194th–343rd amino acid residues at the C-terminal of the protein.The cDNA library of drought stressed cassava leaves was screened by using the 1st–194th amino acid residues at the N-terminal of MeMYB60 protein as bait.Subsequently,18 proteins had been that may interact with MeMYB60.Yeast-two-hybrid analysis determined that MeCatlase1 and MeCataase2 are potential interactors of MeMYB60,respectively.These results lay a foundation for further functional study of MeMYB60 in cassava in response to abiotic stress and are helpful for the regulatory network investigation of MeMYB60.

关 键 词：木薯 非生物胁迫 MYB转录因子 表达特征 互作蛋白筛选 

分 类 号：S533[农业科学—作物学]