患腹泻症状食蟹猴寄生虫和致病菌鉴定与诊断  

Identification and Diagnosis of Parasites and Pathogenic Bacteria in Macaca fascicularis with Diarrhea Symptoms

作　　者：谢永平[1,2] 贺会利 庞彬辉 XIE Yong-ping;HE Hui-li;PANG Bin-hui(Guangxi Veterinary Research Institute,Nanning,Guangxi 530001;Guangxi Key Laboratory of Veterinary Biotechnology,Nanning,Guangxi 530001)

机构地区：[1]广西兽医研究所,广西南宁530001 [2]广西兽医生物技术重点实验室,广西南宁530001

出　　处：《安徽农业科学》2023年第2期104-107,共4页Journal of Anhui Agricultural Sciences

基　　金：广西科学研究与技术开发项目(桂科AD20159087);广西自然科学基金项目(2020GXNSFBA297141);基本科研业务费专项课题(桂科专项21-5)。

摘　　要：[目的]了解广西食蟹猴出现消瘦、腹泻、血便等症状的病因。[方法]采集20份食蟹猴粪便样本,进行寄生虫检测、分子生物学检测和细菌分离鉴定,并对分离菌进行动物致病性和药敏试验。[结果]20份粪便样本均检测出大肠杆菌,12份粪便样本检测出肺炎克雷伯氏菌,10份粪便样本检测出结肠小袋纤毛虫。10份粪便样本为三重感染,2份粪便样本为细菌二重感染。大肠杆菌和肺炎克雷伯氏菌均可导致小鼠死亡,且2种分离菌对阿米卡星、头孢他啶、头孢西丁、头孢噻呋钠均高敏。[结论]结肠小袋纤毛虫、大肠杆菌和肺炎克雷伯氏菌混合感染是引起食蟹猴消瘦、腹泻、血便的主要原因。[Objective]To investigate the etiology of Macaca fascicularis in Guangxi with the emaciation,diarrhea,bloody stools and other symptoms.[Method]Twenty stool samples of M.fascicularis were collected for the parasites and molecular biology detection,bacterial isolation and identification.And the animal pathogenicity and drug sensitivity tests were performed on the isolated bacteria.[Result]Escherichia coli was detected in 20 stool samples,Klebsiella pneumoniae was detected in 12 stool samples,Balantidium coli was detected in 10 stool samples.Ten stool samples were triple infected,two stool samples were double bacterial infection.Both E.coli and K.pneumoniae could cause the death of mice,and two kinds of isolated bacteria were highly sensitive to amikacin,ceftazidime,cefoxitin and ceftiofur sodium.[Conclusion]The main cause of emaciation,diarrhea and bloody stools of M.fascicularis was the mixed infection of B.coli,E.coli and K.pneumoniae.

关 键 词：食蟹猴 结肠小袋纤毛虫 大肠杆菌 肺炎克雷伯氏菌 混合感染 

分 类 号：S851[农业科学—预防兽医学]