EEF1D基因表达对绵羊羊毛纤维直径和毛囊发育的影响  

The Effect of EEF1D Gene Expression on Wool Fiber Diameters and Hair Follicle Development

作　　者：张敏 杨华[2] 魏巧兰[1,2] 何高明 沈敏[2] ZHANG Min;YANG Hua;WEI Qiaolan;HE Gaoming;SHEN Min(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832000;Xinjiang Academy of Agricultural and Reclamation Science,Shihezi,Xinjiang 832000,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832000 [2]新疆农垦科学院,新疆石河子832000

出　　处：《家畜生态学报》2023年第1期27-31,共5页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金(31760661);兵团中青年科技创新领军人才计划(2019CB019)。

摘　　要：为了探讨真核翻译延伸因子1D基因(eukaryotic translation elongation factor 1 Delta,EEF1D)对羊毛品质和毛囊周期发育的影响,采集中国美利奴羊(新疆军垦型)(细毛羊)的10个脏器组织及3个毛囊发育期的中国美利奴羊(新疆军垦型)和多胎萨福克羊(粗毛羊)的毛样品和皮肤组织,测定和比较分析2个绵羊品种在3个毛囊发育期的羊毛纤维直径,应用qRT-PCR检测EEF1D基因在10个组织以及不同毛囊发育期皮肤组织中的表达水平。研究表明,在3个毛囊发育期,中国美利奴羊的羊毛纤维直径均极显著小于多胎萨福克羊(P<0.01)。EEF1D基因在中国美利奴(新疆军垦型)皮肤组织中的表达丰度显著高于其他9个组织(P<0.05)。在毛囊生长期和退行期,EEF1D基因在细毛羊中的表达量显著高于粗毛羊(P<0.05),EEF1D基因的高表达诱导毛囊发育进入休止期。This study aimed to explore the expression of EEF1D gene on wool follicle growth cycle and wool quality of sheep.Ten visceral tissues of Chinese Merino(Xinjiang Junken type)(fine wool sheep)were collected,three wool and skin tissues of Chinese Merino and Prolific Suffolk sheep(course wool sheep)which were in the process of hair follicle development were collected.The wool fiber diameter of the two breeds at three stages of wool follicle development was measured by microscope,and the expression level of EEF1D gene in 10 tissues and skin tissues at different wool follicle development stages was detected by real-time quantitative PCR.Results showed that the fiber diameter of Chinese Merino was extremely lower than that of Prolific Suffolk sheep at three stages of wool follicle development(P<0.01).The expression abundance of EEF1D gene in skin tissues of Chinese Merino(Xinjiang Junken type)was significantly higher than that in other nine tissues(P<0.05).In anagen and catagen phases,the expression level of EEF1D gene in the fine wool sheep was significantly higher than that of coarse wool sheep(P<0.05).The high level expression of EEF1D gene,therefore,induces telogen of hair follicle.

关 键 词：EEF1D基因 基因表达 毛囊发育 纤维直径 绵羊 

分 类 号：S826[农业科学—畜牧学]