大黄鱼养殖海域沉积物中抗生素抗性基因分布特征及其影响因素  

Distribution characteristics and influencing factors of antibiotic resistance genes in sediments from large yellow croaker(Larimichthys crocea)culture areas

作　　者：黄薇 刘洋[1] 饶秋华 罗钦 王为刚 宋永康 罗土炎 HUANG Wei;LIU Yang;RAO Qiuhua;LUO Qin;WANG Weigang;SONG Yongkang;LUO Tuyan(Institute of Agricultural Quality Standards and Testing Technology Research,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality&Safety,Fuzhou 350003,China;College of Life Science,Fujian Normal University,Fuzhou 350108,China;Aquatic Technology Promotion Station of Lianjiang County,Fuzhou 350500,China)

机构地区：[1]福建省农业科学院农业质量标准与检测技术研究所/福建省农产品质量安全重点实验室,福州350003 [2]福建师范大学生命科学学院,福州350108 [3]连江县水产技术推广站,福州350500

出　　处：《农业环境科学学报》2023年第1期197-208,共12页Journal of Agro-Environment Science

基　　金：福建省属公益类科研院所基本科研专项(2020R1022009,2022R1022002);福建省农业科学院科技项目(YC2019011,2022KF20,CXTD2021015-3)。

摘　　要：为揭示大黄鱼养殖海域沉积物中抗生素抗性基因(Antibiotic resistance genes,ARGs)的赋存特征及其影响因素,本研究通过野外调查采集了6个站点的大黄鱼养殖海域沉积物样品,利用宏基因组测序分析技术解析沉积物中ARGs的分布特征,同时运用化学分析技术和高通量测序技术对沉积物中的环境因子以及微生物群落结构进行分析,以进一步对ARGs与环境因子以及微生物群落结构的相关性进行研究。结果显示:大黄鱼养殖海域沉积物中共检出781个ARGs亚型,分属于38种抗生素抗性类型;不同站点的优势ARGs抗生素抗性类型基本一致,主要为四环素类、大环内酯类和氟喹诺酮类,引起细菌耐药性的主要机制为外排泵。进一步相关性分析结果显示,除PNGM-1与电导率和TN呈显著正相关(P<0.05)外,其余丰度前20的ARGs均与环境因子相关性不显著(P>0.05);而丰度前20的ARGs均与丰度前20的菌属呈显著或极显著相关关系(P<0.05或P<0.01)。研究表明,大黄鱼养殖海域沉积物中含有丰富的ARGs,微生物群落是影响沉积物中ARGs分布的重要因素。To reveal the distribution characteristics and influencing factors of antibiotic resistance genes(ARGs)in the sediments of large yellow croaker culture areas,sediment samples from six sites of large yellow croaker culture areas were collected via field survey,and the distribution characteristics of ARGs in the sediments were analyzed using macro-genome sequencing analysis technology.The environmental factors and microbial community structure in the sediment were analyzed using chemical analysis and high-throughput sequencing techniques,and the correlation between ARGs and environmental factors and microbial community structure was investigated.781 ARGs subtypes belonging to 38 ARGs types were identified,indicating the wide-spectrum profiles of ARGs in the sediments from large yellow croaker culture areas.Statistical analyses revealed that the types of antibiotic resistance in the dominant ARGs in sediments from different large yellow croaker culture areas were basically the same,mainly tetracyclines,macrolides,and fluoroquinolones,and the main mechanism causing bacterial resistance was the efflux pump.Correlation analysis showed that the top 20 ARGs were not significantly correlated(P>0.05)with environmental factors,except for PNGM-1,which was significantly and positively correlated(P<0.05)with conductivity and TN;however,all the top 20 ARGs were significantly or highly significantly correlated(P<0.05,P<0.01)with the top 20bacterial genera.Our results indicate that sediments from large yellow croaker culture areas are rich in ARGs,and the microbial community is an important factor that affects the distribution of ARGs in the sediments.

关 键 词：抗生素抗性基因 宏基因组测序分析技术 大黄鱼养殖海域 沉积物 微生物群落 

分 类 号：X55[环境科学与工程—环境工程]