鼠尾振动暴露大鼠血液中前列环素和血栓素的变化  

Expression changes of prostacyclin and thromboxane in blood of rats with tail vibration

作　　者：陈子宇 魏诺言 梁芷珊 黄惠民 胡秀文 王军义 陈青松 杨虹雨 CHEN Ziyu;WEI Nuoyan;LIANG Zhishan;HUANG Huimin;HU Xiuwen;WANG Junyi;CHEN Qingsong;YANG Hongyu(School of Public Health,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510224,China)

机构地区：[1]广东药科大学公共卫生学院,广东广州510224

出　　处：《环境与职业医学》2022年第11期1226-1230,共5页Journal of Environmental and Occupational Medicine

基　　金：广东省自然科学基金面上项目(2022A1515011357,2022A1515012412);广东省基础与应用基础区域联合基金青年基金项目(2019A1515110492);广州市科技计划项目(201904010222)。

摘　　要：[背景]长期接触手传振动暴露会导致手臂振动综合征,表现之一是手臂外周血液循环障碍。血中前列环素I2(PGI2)及血栓素A2(TXA2)表达改变可能是振动致手臂振动综合征的重要机制之一。[目的]揭示大鼠鼠尾接触振动对血浆中PGI2、TXA2表达量的影响,探讨大鼠血浆中二者比值变化与鼠尾振动的关联。[方法]50只SPF级雄性SD大鼠,利用随机区组法分为对照组、1 d接振组、3 d接振组、7 d接振组、14 d接振组共5组,每组10只;大鼠放置于大鼠固定器中,摆放到固定台相应位置,大鼠尾巴置于振动仪用医用胶带固定好,固定台与振动仪不接触,使固定器之间、鼠尾之间不重叠;暴露剂量为125 Hz、5.9 m·s^(-2)、4 h·d^(-1),振动方向是线性垂直振动;振动暴露结束取腹主动脉血,利用酶联免疫吸附试验检测血管因子PGI2、TXA2及其水解产物6-酮-前列腺素F1α(6-keto-PGF1α)、血栓素B2(TXB2)的表达量,并计算6-keto-PGF1α/TXB2值。利用Spearman秩相关分析血管因子的表达量是否与振动累积时间相关。[结果]1 d组、3 d组、7 d组、14 d组大鼠血浆6-keto-PGF1α的表达量分别为(896.12±124.37)、(1068.13±119.41)、(1215.26±122.64)、(1317.94±106.54)ng·L^(-1),均较对照组的(830.60±109.47)ng·L^(-1)升高(P<0.001);PGI2表达量分别为(86.49±2.40)、(107.90±2.65)、(114.02±2.16)、(126.95±1.94)ng·L^(-1),均较对照组的(60.09±2.11)ng·L^(-1)升高(P<0.001);TXB2的表达量分别为(132.14±4.10)、(145.52±4.09)、(179.91±4.98)、(204.10±3.22)ng·L^(-1),均较对照组的(106.08±3.26)ng·L^(-1)升高(P<0.001)。3 d组、7 d组、14 d组大鼠血浆TXA2的表达量分别为(211.99±3.24)、(236.33±3.88)、(245.45±4.23)ng·L^(-1),均较对照组的(174.79±4.19)ng·L^(-1)升高(P<0.001)。与对照组相比,7 d组、14 d组大鼠6-keto-PGF1α/TXB2值均下降(P<0.05)。6-ketoPGF1α、PGI2、TXB2、TXA2表达量均与振动累积时间呈正相关(r=0.84、0.84、0.80、0.84,P<0.001),6[Background]Long-term exposure to hand-transmitted vibration can lead to hand-arm vibration syndrom e,one manifestation of which is impaired pe ripheral blood circulation in the arms.Altered expressions of prostacyclin I2(PGI2)and thromboxane A2(TXA2)in blood may be one of the important mechanisms of vibration-induced hand-arm vibration syndrome.[Objective]To reveal the effects of rat tail vibration on the expressions of PGI2and TXA2in plasma,and to establish the correlation between the change of rat plasma PGI2to TXA2ratio and rat tail vibration.[Methods]Fifty SPF-grade male SD rats were randomly divided into five groups:control group,1 d exposure group,3 d exposure group,7 d exposure group,and 14 d exposure group,with 10 rats in each group.The rats were placed in rat immobilizes on a immobilization table,and the rats’tails were connected to a shaker and fixed with medical tape.There was no overlap between the immobilizes and between the rats’tails by no contact between the immobilization table and the shaker.The exposure dose was 125 Hz,5.9 m·s^(-2),4 h·d^(-1),and the vibration direction was linear vertical vibration.Abdominal aortic blood was taken at the end of vibration exposure,and the expressions of PGI2,TXA2,and their hydrolysates 6-keto-prostaglandin F1α(6-keto-PGF1α)and thromboxane B2(TXB2)were measured by enzyme-linked immunosorbent assay,and the 6-keto-PGF1α/TXB2values were calculated.Spearman rank correlation was used to analyze whether the expression of vascular factors correlated with the accumulated time of vibration.[Results]The expression levels of plasma 6-keto-PGF1αwere(896.12±124.37),(1068.13±119.41),(1215.26±122.64),and(1317.94±106.54)ng·L^(-1)in the 1 d,3 d,7 d,and 14 d groups of rats,respectively,which were higherthan that in the control group,(830.60±109.47)ng·L^(-1)(P<0.001).The PGI2expression levels were(86.49±2.40),(107.90±2.65),(114.02±2.16),and(126.95±1.94)ng·L^(-1)in the 1 d,3 d,7 d,and14 d groups of rats,respectively,all higher than(60.09±2.11)ng·L^(-1)in th

关 键 词：鼠尾振动 大鼠鼠尾振动模型 前列环素 血栓素 

分 类 号：R13[医药卫生—劳动卫生]