鸡NRAMP1蛋白序列特征、分子进化与突变分析  

Characterization,Molecular Evolution and Mutation Analysis on Chicken Natural-Resistance-Associated Macrophage Protein 1(NRAMP1)

作　　者：见玉文 王金萍 宁波 薛振 胡媛媛 王珊珊 王令[1,3,4] JIAN Yuwen;WANG Jinping;NING Bo;XUE Zhen;HU Yuanyuan;WANG Shanshan;WANG Lin(College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;Shaanxi Province Key Laboratory of Bio-Resources,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C.,Hanzhong,Shaanxi 723001;Qinba State Key Laboratory of Biological Resources and Ecological Environment,Shaanxi University of Technology(Cultivation),Hanzhong,Shaanxi 723001)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723001 [2]陕西理工大学陕西省资源生物重点实验室,陕西汉中723001 [3]陕南秦巴山区生物资源综合开发协同创新中心,陕西汉中723001 [4]陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室(培育),陕西汉中723001

出　　处：《中国家禽》2023年第1期23-31,共9页China Poultry

基　　金：陕西省教育厅重点科学研究计划项目(20JY006);陕西省科技厅重点研发项目(2020NY-003)。

摘　　要：为解析鸡天然抗性相关巨噬蛋白1(Natural-resistance-associated macrophage protein 1,NRAMP1)223位Arg突变为Gln对蛋白质结构和理化性质的影响,试验系统分析了鸡NRAMP1蛋白质结构特征,重点比较鸡NRAMP1223位Arg(抗性)和Gln(易感)的蛋白质理化性质和结构差异,并分析略阳乌鸡NRAMP1基因该位点的多态性。结果表明,NRAMP1蛋白质保守性较高,鸡和人NRAMP1氨基酸序列相似度达68%,以鸡NRAMP1的第223位氨基酸为参考,其他物种该位点均为碱性氨基酸(Arg、Lys、His);鸡NRAMP1第2454位G突变为A,223位Arg变成Gln,其理化性质和二、三级结构均发生变化,该位点附近的β-转角变为α-螺旋。略阳乌鸡NRAMP1基因该位点的GG基因型频率为67.1%,GA频率30.1%,AA频率2.7%。研究结果证实鸡NRAMP1蛋白质223位突变改变其结构和理化性质,可能与该蛋白抗感染能力相关。In order to analyze the effect of mutation to Gln in chicken natural resistance-associated macrophage protein 1(NRAMP1)223 site on protein structure and physicochemical properties,the structural characteristics of NRAMP1 were systematically analyzed.The differences of Arg(resistance)and Gln(susceptibility)on the physicochemical properties and structure of chicken NRAMP1 were compared,and the polymorphism of NRAMP1 gene at this was analyzed in Lueyang black-bone chicken.The results showed that NRAMP1 protein was highly conserved,and the similarity of amino acids between chicken and human was 68%.Taken the 223rd amino acid of chicken NRAMP1 as reference,this site was basic amino acids(Arg,Lys,His)in other species.In chicken NRAMP1,G in 2454 site mutated into A,and the corresponding arginine(Arg)changed into glutamine(Gln).Based on the mutation,the physicochemical properties,secondary and tertiary structure of NRAMP1 were altered,and the β-turn near the mutation site changed toα-helix.Based on The GG genotype frequency of NRAMP1 gene was 67.1%,GA frequency was 30.1%,AA frequency was 2.7% in Lueyang black-bone chicken,respectively.This study demonstrated that the structure and physicochemical properties of chicken NRAMP1 were altered by mutation of Arg to Gln in 223 site,which might be related to its anti-infection ability.

关 键 词：NRAMP1 基因多态性 抗感染能力 略阳乌鸡 

分 类 号：S831.2[农业科学—畜牧学]