新型鹅细小病毒信阳株的全基因组扩增及遗传进化分析  

Whole genome amplification and phylogenetic analysis of novel goose parvovirus Xinyang strain

作　　者：焦凤超[1] 张鑫 李迎晓[1] 雷震 曲哲会[1] 董建国[1] 赵瑜[1] 何书海[1] 李洵[1] 黄立[1] 赵聘[1] JIAO Fengchao;ZHANG Xin;LI Yingxiao;LEI Zhen;QU Zhehui;DONG Jianguo;ZHAO Yu;HE Shuhai;LI Xun;HUANG Li;ZHAO Pin(Xinyang Agricultural and Forestry University/Engineering Technology Research Center for Waterfowl Resources Development and Utilization and Epidemic Disease Prevention and Control of Henan Province,Xinyang 464000,China)

机构地区：[1]信阳农林学院/河南省水禽资源开发利用与疫病防控工程技术研究中心,河南信阳464000

出　　处：《畜牧与兽医》2023年第1期112-119,共8页Animal Husbandry & Veterinary Medicine

基　　金：河南省科技攻关项目(222102110188);信阳市创新应用专项(20200016);信阳农林学院重要水禽源病原致病与免疫机制研究科技创新团队项目;信阳农林学院家禽疫病防控科技服务团队项目。

摘　　要：为了解信阳地区新型鹅细小病毒(NGPV)的遗传变异特性,对疑似NGPV感染麻鸭病例进行病原检测,采集肝脏和脾脏无菌处理后接种SPF鸭胚,成功分离到1株NGPV,命名为HNXY21株,并对其进行全基因组序列分析和VP1基因重组鉴定。序列比对和系统发育树表明,分离株HNXY21和其他9株NGPV同处于鹅细小病毒(GPV)组内的一个独立分支上,与山东分离株SD0316(GenBank登录号:MN415969)核苷酸同源性最高;VP1蛋白的氨基酸序列分析显示,与经典GPV和番鸭细小病毒(MDPV)相比,有8个氨基酸位点突变;通过Simplot软件对VP1基因进行重组分析表明,分离株HNXY21 VP1基因存在重组信号,其潜在的主要亲本为山东分离株SD0316和弱毒疫苗株SYG61v。本研究结果丰富了NGPV的分子生物学资料,为进一步研究NGPV的致病机制奠定了基础。In order to understand the genetic variation characteristics of a novel goose parvovirus(NGPV) in the Xinyang area, the pathogen detection of suspected hemp duck cases was carried out. After the liver and spleen samples collected from the ducks were aseptically treated, the resulting supernatant was inoculated with a specific pathogen free duck embryos, and a NGPV strain was successfully isolated and was named as HNXY21. Then, whole genome sequence analysis and recombination identification were carried out. The sequence alignment and phylogenetic analysis showed that the isolate HNXY21 and the other 9 NGPV isolates were in an independent branch within the GPV group, and they had the highest homology with the Shandong isolate SD0316(MN415969). The amino acid sequence analysis of VP1 proteins showed that there were eight typical amino acid variation sites. One recombination signal was redetected in the VP1 gene of HNXY21 using the Simplot software, which showed that the potential parents of the isolate HNXY21 were the Shandong isolate SD0316(MN415969) and the vaccine strain of SYG61 v. The results of this study enriched the molecular biological data of NGPV and laid a foundation for further research on the pathogenic mechanism of NGPV.

关 键 词：新型鹅细小病毒 遗传变异 重组 

分 类 号：S855.3[农业科学—临床兽医学]