Plackett-Burman设计结合Box-Behnken响应面法优化紫斑牡丹籽壳中低聚芪类和单萜苷类化合物的超声提取工艺  

Optimization of the Ultrasonic Extraction Process of the Seed Coat from Paeoniarockii(S.G.Haw.et.Laeuner)T.Hanget.T.J.Li by Plackett-Burman Design Combined with Box-Behnken Response Surface Method

作　　者：李洁 石晓峰[1,2,3] 叶倩女 董学凤[2] 张家旭 马趣环 刘东彦 LI Jie;SHI Xiaofeng;YE Qiannyu;DONG Xuefeng;ZHANG Jiaxu;MA Quhuan;LIU Dongyan(Lanzhou Institute for Food and Drug Control,Gansu Provincial Engineering Research Center for Monitoring Exogenous Pollutants of Planting Chinese Medicinal Materials,Lanzhou 730050,China;Gansu University of Chinese Medicine,Lanzhou 730030,China;Gansu Academy of Medical Science,Lanzhou 730050,China)

机构地区：[1]兰州市食品药品检验检测研究院,甘肃省种植中药材外源性污染物监测工程研究中心,兰州730050 [2]甘肃中医药大学,兰州730030 [3]甘肃省医学科学研究院,兰州730050

出　　处：《中国现代应用药学》2023年第1期68-74,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：甘肃省药品监督管理局2022年度科研计划项目(2022GSMPA0070);甘肃省省级中医药科研课题(GZK-2017-44)。

摘　　要：目的优化紫斑牡丹籽壳中低聚芪类和单萜苷类化合物的超声提取工艺。方法以HPLC测得的芍药苷和白藜芦醇得率为指标,采用综合评分法,在提取功率、液料比、提取温度、提取时间、乙醇浓度和提取次数6个单因素试验的基础上,通过Plackett-Burman试验从中遴选显著因素,进而采用Box-Behnken响应面法优化紫斑牡丹籽壳中低聚芪类和单萜苷类化合物的超声提取工艺。结果优化得到的超声提取工艺为乙醇浓度75%、液料比30 mL·g^(–1)、提取功率210 W、温度50.0℃、提取50 min、提取2次。在此最佳工艺下,芍药苷和白藜芦醇的得率分别为5472.43,1633.11μg·g^(–1),实测值与预测值基本相符。结论优化得到的紫斑牡丹籽壳中低聚芪类和单萜苷类化合物的超声提取工艺合理,可为其后续的应用研究提供参考。OBJECTIVE To optimize the ultrasonic extraction technology of oligostilbene and monoterpene glycosides from the seed coat of Paeonia rockii(S.G.Haw.et.Laeuner)T.Hanget.T.J.Li.METHODS Take paeoniflorin and resveratrol measured by HPLC as indicators,using comprehensive scoring method,on the basis of 6 single factor experiments of extraction power,liquid-to-material ratio,extraction temperature,extraction time,ethanol concentration and extraction times,select significant factors by the Plackett-Burman test,and then optimize the ultrasonic extraction process of oligostilbene and monoterpene glycosides from the seed coat of Paeonia rockii(S.G.Haw.et.Laeuner)T.Hanget.T.J.Li by the Box-Behnken response surface method.RESULTS The optimized ultrasonic extraction process was as follows:ethanol concentration 75%,liquid-to-material ratio 30 mL·g^(–1),ultrasonic power 210 W,temperature 50.0℃,extraction 50 min,extraction 2 times.Under this optimal process,the contents of paeoniflorin and resveratrol were 5472.43 and 1633.11μg·g^(–1),and the measured values were basically in agreement with the predicted values.CONCLUSION The optimized ultrasonic extraction process of oligostilbene and monoterpene glycosides from the seed coats of Paeonia rockii(S.G.Haw.et.Laeuner)T.Hanget.T.J.Li is reasonable,it can provide reference for its follow-up application research which can provide a reference for its subsequent application research.

关 键 词：紫斑牡丹籽壳 超声提取工艺 芍药苷 白藜芦醇 Plackett-Burman试验 Box-Behnken响应面法 

分 类 号：R285.5[医药卫生—中药学]