痛泻要方合四逆散对糖尿病KK-Ay小鼠胰岛素抵抗及PI3K/AKT/GSK-3β通路的影响  

Effects of Tongxie Yaofang and Sini Powder on PI3K/AKT/GSK-3βsignal pathway and insulin resistance in KK-Ay mice

作　　者：胡浩 贾晓蕾 HU Hao;JIA Xiaolei(Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China;The Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学东方医院内分泌科,北京100078 [2]北京中医药大学第三附属医院,北京100029

出　　处：《天津中医药》2023年第2期214-218,共5页Tianjin Journal of Traditional Chinese Medicine

基　　金：北京中医药大学青年教师项目(2018-JYBZZ-JS176)。

摘　　要：[目的]观察痛泻要方合四逆散对KK-Ay小鼠胰岛素抵抗及磷脂酰肌醇3-激酶/蛋白激酶B/糖原合成酶激酶-3β(PI3K/AKT/GSK-3β)信号通路的影响。[方法]采用雄性6~8周龄KK-Ay小鼠建立糖尿病模型,随机分为模型组、中药低中高不同剂量组,另取同周龄C57 BL/6J小鼠作为正常组,干预4周后,检测小鼠空腹血糖,空腹胰岛素水平及胰岛功能情况,并通过实时荧光定量反转录聚合酶链式反应(real time PCR),蛋白免疫印迹(Western Blot)等方法检测小鼠肝脏中PI3K、AKT、GSK-3β的基因及蛋白磷酸化水平表达情况。[结果]与正常组相比,模型组小鼠空腹血糖和胰岛素抵抗指数明显升高(P<0.05),而PI3K、AKT、GSK-3β的磷酸化蛋白水平及基因表达水平均明显降低(P<0.05);与模型组相比,中药高剂量组空腹血糖和胰岛素抵抗指数明显降低(P<0.05),而PI3K、AKT、GSK-3β的磷酸化蛋白水平及基因表达水平均明显升高(P<0.05)。[结论]痛泻药方合四逆散可能通过影响PI3K/AKT/GSK-3β信号通路改善KK-Ay小鼠的胰岛素抵抗。[Objective]To observe the effects of Tongxie Yaofang and Sini Powder on PI3K/AKT/GSK-3βsignal pathway and insulin resistance in KK-Ay mice.[Methods]Male 6 to 8 week old KK-Ay mice were used to establish a diabetes model.They were randomly divided into model group,and low,medium,high dosage groups of Chinese medicine.C57BL/6J mice of the same week were taken as the normal group.After 4 weeks of intervention,fasting insulin levels,insulin and islet function of mice were detected,and the protein phosphorylation and mRNA expression of PI3K,AKT and GSK-3βin mice liver were detected by real time PCR and Western blot.[Results]Compared with the normal group,the fasting blood glucose and insulin resistance index of the model group mice were significantly increased(P<0.05),while the phosphorylated protein levels and gene expression levels of PI3K,AKT,GSK-3βwere significantly reduced(P<0.05).Compared with the model group,the fasting blood glucose and insulin resistance index in the high dose group were significantly reduced(P<0.05),while the phosphorylated protein levels and gene expression levels of PI3K,AKT,GSK-3βwere significantly increased(P<0.05).[Conclusion]Tongxie Yaofang and Sini Powder may improve insulin resistance in KK-Ay mice by affecting PI3K/AKT/GSK-3βsignaling pathway.

关 键 词：痛泻药方 四逆散 胰岛素抵抗 PI3K/AKT/GSK-3β信号通路 

分 类 号：R587.1[医药卫生—内分泌]