JAK/STAT信号通路在大鼠重症急性胰腺炎早期作用机制的研究  

Mechanism of JAK/STAT signaling pathway in the early stage severe acute pancreatitis rats

作　　者：程峰[1] 邱兆磊[1] 郑传明[1] 夏雅琴 李磊[1] 杜召辉[1] 姜海[1] 王振杰[1] 解福臣[1] CHENG Feng;QIU Zhao-lei;ZHENG Chuan-ming;XIA Ya-qin;LI Lei;DU Zhao-hui;JIANG Hai;WANG Zhen-jie;XIE Fu-chen(Department of Emergency Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China;不详)

机构地区：[1]蚌埠医学院第一附属医院急诊外科,安徽蚌埠233004 [2]蚌埠医学院临床医学一系,安徽蚌埠233003

出　　处：《中华全科医学》2023年第1期41-44,65,共5页Chinese Journal of General Practice

基　　金：安徽省教育厅重点项目(KJ2019A0385);蚌埠医学院自然科学基金项目(BYKY1779);安徽省大学生创新创业训练计划项目(S201910367121);蚌埠医学院自然科学重点项目(BYKY2019095ZD,2021byzd160)。

摘　　要：目的探讨抑制JAK/STAT信号通路对大鼠重症急性胰腺炎(SAP)早期相关疾病指标的影响,从而推测该信号通路在SAP大鼠中可能的作用机制。方法将54只成年SD大鼠按随机数字表分成对照组、SAP组、AG490组,每组18只,AG490组造模前给予AG490,其他组给予等量生理盐水,以5%牛磺胆酸钠逆行胆胰管内注射法制作SAP模型,造模后6、12、24 h每组分别处死6只大鼠,取心房血测血淀粉酶、血钙、TNF-α、IL-1、IL-6,留取胰腺组织行HE染色病理检查及Western blotting法检测胰腺组织中STAT3蛋白表达水平。结果AG490组在6、12、24 h淀粉酶值分别为(2049.17±257.00)U/L、(2915.00±188.42)U/L、(3746.50±181.05)U/L,高于对照组、低于SAP组,SAP组高于对照组(均P<0.05);AG490组6、12、24 h血钙浓度高于SAP组,2组均低于对照组(均P<0.05);AG490组6、12、24 h TNF-α、IL-6、IL-1均低于SAP组,2组均高于对照组;SAP组胰腺组织STAT3表达量随时间增加,24 h明显高于AG490组,其他时间点差异不明显。SAP组、AG490组胰腺病理切片可见胰腺细胞水肿、炎性细胞浸润,差异不明显。结论SAP早期阶段可能是通过JAK/STAT信号通路诱导多种炎性因子分泌,促进胰腺炎的进展。Objective To investigate the effect of inhibiting JAK/STAT signaling pathway on relevant disease indicators in rats with early stage severe acute pancreatitis(SAP),so as to speculate the possible mechanism of this signaling pathway in SAP rats.Methods Fifty-four adult SD rats were divided into control group,SAP group and AG490 group according to random number table,18 rats in each group.AG490 group was given AG490 application before modeling,other groups were given equal amount of saline.SAP model was made by retrograde intra-biliary pancreatic duct injection with 5%sodium taurocholate,and the rats were executed at 6 h,12 h and 24 h after modeling,and atrial blood was taken to measure serum amylase,calcium,TNF-αand calcium.The pancreatic tissues were examined by HE staining.Western blotting was used to detect the expression level of STAT3 protein in pancreatic tissues.Results The amylase values in the AG490 group at 6 h,12 h and 24 h were(2049.17±257.00)U/L,(2915.00±188.42)U/L and(3746.50±181.05)U/L,respectively,which were higher than those in the control group and lower than those in the SAP group,and SAP group was higher than the control group(all P<0.05).The blood calcium concentrations at 6 h,12 h and 24 h in the AG490 group were higher than those in the SAP group,both groups were lower than the control group(all P<0.05).The TNF-α,IL-6 and IL-1 at 6 h,12 h and 24 h in the AG490 group were lower than those in the SAP group,and both groups were higher than the control group.STAT3 expression in SAPgrouppancreatic tissue increased with time between groups,and the 24 h point was significantly higher than the AG490 group,with insignificant differences at other points.AG490 group,pancreatic cell edema and inflammatory cell infiltration were seen in pathological sections,no significant difference.Conclusion The early stage of severe acute pancreatitis may induce multiple inflammatory factors through the JAK/STAT signaling pathway and promote the progression of pancreatitis.

关 键 词：重症急性胰腺炎 酪氨酸蛋白激酶 信号转导及转录激活因子 信号通路 

分 类 号：R657.51[医药卫生—外科学] R-332[医药卫生—临床医学]